金属铜配合物的合成、晶体结构、抗癌活性及与牛血清白蛋白的相互作用（英文）  

Synthesis, Crystal Structure, Anticancer Activity and Interaction with Bovine Serum Albumin of Copper(Ⅱ) Complex

作　　者：张燕[1] 孟祥高[2] 蔡苹[3] 程功臻[3] 贾士芳[1] ZHANG Yan;MENG Xiang-Gao;CAI Ping;CHENG Gong-Zhen;JIA Shi-Fang(School of Chemical and Biological Engineering,Taiyuan Science and Technology University,Taiyuan 030021,China;College of Chemistry,Central China Normal University,Wuhan 430079,China;College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China)

机构地区：[1]太原科技大学化学与生物工程学院,太原030024 [2]华中师范大学化学学院,武汉430079 [3]武汉大学化学与分子科学学院,武汉430072

出　　处：《无机化学学报》2018年第10期1864-1874,共11页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21101121);太原科技大学博士启动基金(No.20172005)资助~~

摘　　要：合成了一个含有席夫碱配体的四配位的铜配合物,通过元素分析、红外光谱对其进行表征并通过X射线单晶衍射仪测试它的结构,属于单斜晶系。此外,通过MTT法(MTT为3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐)测试了配合物对宫颈癌细胞、胃癌细胞、肝癌细胞和乳腺癌细胞的细胞毒性。结果表明铜配合物对4种肿瘤细胞的活性比顺铂高。通过荧光光谱法研究了配合物与牛血清白蛋白的相互作用,荧光光谱表明配合物与血清结合过程中发生了静态猝灭,并且计算了结合常数、结合位点数、结合距离和热力学常数ΔH,ΔS和ΔG。A four-coordinated Cu(Ⅱ)complex with Schiff base ligand was synthesized and characterized by the elemental analysis and infrared spectroscopy,and the crystal structure was determined by X-ray diffraction.The crystal of complex belongs to monoclinic system.In vitro antitumor activities of Cu(Ⅱ)complex were evaluated by the 3-(4,5-dimethylthiazoly-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)assay against four human cancer cell lines(Hela,SGC-7901,HepG2,MCF-7).Cu(Ⅱ)complex exhibited stronger antitumor activity than carboplatin.The interaction of Cu(Ⅱ)complex with bovine serum albumin(BSA)was studied by spectroscopic method.The analysis of fluorescence data indicates the presence of static quenching mechanism in the binding process.Various binding parameters including binding constants,binding sites,binding distance and thermodynamic parametersΔH,ΔS andΔG have been evaluated.CCDC:1567198.

关 键 词：铜配合物 晶体结构 抗癌活性 牛血清白蛋白 荧光光谱 

分 类 号：O614.121[理学—无机化学]