福建省53份茶树种质资源SSR-PCR反应体系的优化  

Optimization of SSR-PCR Reaction System for 53 Tea (Camellia sinensis) Germplasm Resources in Fujian Province

作　　者：朱晨[1,2] 田采云 张舒婷 常笑君[1,2] 傅海峰[1] 李小桢 陈常颂 林玉玲 郭玉琼[1,2] ZHU Chen;TIAN Cai-yun;ZHANG Shu-ting(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;Key Laboratory of Tea Science in Fujian Province,Fuzhou,Fujian 350002;Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]茶学福建省高校重点实验室,福建福州350002 [3]福建农林大学园艺植物生物工程研究所,福建福州350002 [4]福建省农业科学院茶叶研究所,福建福安355015

出　　处：《安徽农业科学》2018年第28期88-91,共4页Journal of Anhui Agricultural Sciences

基　　金：福建省重大科技专项(2015NZ0002-1);福建省重大农技推广项目(KNJ151003);福建农林大学科技创新专项(CXZX2017164);福建农林大学乡村振兴茶产业技术服务项目(11899170102)

摘　　要：[目的]优化茶树种质资源SSR-PCR反应体系。[方法]以福建省不同地区的53份茶树种质资源作为SSR-PCR体系优化的供试材料,通过L16(43)正交试验设计,对茶树SSR-PCR反应体系的3个影响因素:DNA模板浓度(ng/μL)、引物浓度(mmol/L)和Taq酶浓度(U)在4个水平上进行比较筛选,并进行PCR验证。[结果]优化后最佳的福建省茶树SSR-PCR反应体系为25μL体系,其中DNA模板浓度50 ng/μL、引物浓度0.25 mmol/L、Taq酶浓度1.25 U。并从23对茶树SSR引物中筛选出5对清晰、明亮、无杂带的引物,适用于福建省茶树SSR标记的进一步研究。[结论]该研究可为后续福建省茶树品种的亲缘关系分析、杂交品种的选育等研究提供依据。[Objective]To optimize the SSR-PCR reaction system for tea germplasm resources.[Method]Fifty-three tea plant germplasm resources in different regions of Fujian Province were used as the optimized materials for the SSR-PCR system.Through the L 16(4 3)orthogonal design,three factors affecting the SSR-PCR reaction system of tea plant,DNA template concentration(ng/μL),primer concentration(mmol/L)and Taq enzyme concentration(U)were screened at four levels,and PCR verification was performed.[Result]The optimum SSR-PCR reaction system for tea in Fujian Province was 25μL system,in which the concentration of DNA template was 50 ng/mL,primer concentration was 0.25 mmol/L,Taq enzyme concentration was 1.25 U.From 23 pairs of SSR primers,5 pairs of primers with clear,bright and no-stray bands were selected,which is suitable for further study on SSR markers of tea plants in Fujian Province.[Conclusion]This study provides a basis for the analysis of the genetic relationship and hybrid breeding of tea cultivars in Fujian Province

关 键 词：茶树 种植资源 SSR-PCR 体系优化 正交设计 

分 类 号：S571.1[农业科学—茶叶生产加工]