5 L发酵罐中棘白菌素B发酵工艺参数优化  

Optimization of the fermentation parameters for Echinocandin B production in 5 L bioreactor

作　　者：胡晓龙 秦海彬[1] 吴旭萍 毛健[1] 邹树平[1] 牛坤[1] HU Xiao-long;QIN Hai-bin;WU Xu-ping;MAO Jian;ZOU Shu-ping;NIU Kun(Key Laboratory of Bioorganic Synthesis of Zhejiang Province,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江工业大学浙江省生物有机合成研究重点实验室,浙江杭州310014

出　　处：《食品与发酵工业》2018年第9期130-135,共6页Food and Fermentation Industries

摘　　要：棘白菌素B(Echinocandin B,ECB)是抗真菌药物阿尼芬净的前体物质,主要通过微生物发酵合成。实验对构巢曲霉(Aspergillus nidulans ZJB09223)在5 L发酵罐中的培养条件进行了初步探索,考察了不同的搅拌桨类型、搅拌转速以及通气量等对棘白菌素B发酵过程的影响,为实现阿尼芬净的工业化大规模生产探明基本条件。结果表明,与双折叶式搅拌桨和框式搅拌桨相比,六直叶圆盘涡轮搅拌桨更适合ECB的发酵过程。在5 L机械搅拌式发酵罐中,最佳通气量为1. 7 VVM,在发酵前6 d,搅拌转速控制为600 r/min,后9 d搅拌转速控制为400 r/min,可以提高ECB的发酵产量。在该工艺条件下ECB发酵产量为608 mg/L,比优化前提高了193%。Echinocandin B(ECB)is the key precursor of anidufungin,mainly synthesized by microbial fermentation.In this experiment,the scale-up conditions of Aspergillus nidulans ZJB09223 for ECB fermentation was optimized in the fermentor.The effects of different types of agitating paddles,stirring speed and aeration volume on the Echinocandin B fermentation were investigated to achieve the basic conditions of industrialization.The comparison of different impeller types showed that flat-straight leaf disc tubine type agitator was more suitable for ECB fermentation process.The optimum aeration amount was 1.7 VVM,and the stirring speed was 600 r/min in the first 6 days and 400 r/min in the next 9 days to obtain the best results of 608 mg/L,which was 193%higher than that under the original conditions.

关 键 词：构巢曲霉 棘白菌素B 搅拌桨类型 通气量 搅拌转速 

分 类 号：TQ927[轻工技术与工程—发酵工程]