不产毒黄曲霉菌株的筛选鉴定及分子机理研究  

Screening and Mechanism Study on an Aspergillus flavu Strain Which cannot Produce Aflatoxin

作　　者：刘俊 张国朋 张智猛[2] 冯东晓[3] LIU Jun;ZHANG Guo-peng;ZHANG Zhi-meng;FENG Dong-xiao(Qingdao Zhicheng Biological Technology Co.Ltd,Qingdao 266101,China;Peanut Research Institute, Qingdao 266100,China;The University of Binzhou Medical School,Yantai 264003,China)

机构地区：[1]青岛至成生物技术有限公司,山东青岛266101 [2]山东省花生研究所,山东青岛266100 [3]滨州医学院药学院,山东烟台264003

出　　处：《花生学报》2018年第3期8-13,共6页Journal of Peanut Science

基　　金：青岛市民生科技计划项目(17-3-3-56-nsh)

摘　　要：本研究经过筛选分离得到一种不产毒的黄曲霉菌株CGMCC NO.14122,经产毒鉴定,该菌株在生长过程中不产生黄曲霉毒素;DNA序列分析表明,该菌的AflR基因启动子序列发生了较长片段的缺失突变。该菌株在生长和产孢能力上与野生型产毒黄曲霉菌无差别,证明该黄曲霉菌可以用于黄曲霉毒素污染的生物防控。An Aspergillus flavus stain that cannot produce aflatoxin has been characterized.Long deletion was identified in the promoter region of AflR gene of the strain by DNA sequence analysis,thus all aflatoxin synthesis related genes couldn't be initiated,therefore the strain cannot produce aflatoxin.The strain can compete with the toxin-producing Aspergillus flavus in the soil,we have proved that this Aspergillus flavus strain can be used for the prevention and control of aflatoxin pollution and it has good bio-control effect.

关 键 词：黄曲霉毒素 黄曲霉菌 基因突变 DNA序列 

分 类 号：Q93-331[生物学—微生物学] Q754