核磁共振法测定蛋黄中磷脂酰胆碱含量的实验研究  

Experimental study on determination of phosphatidylcholine content in egg yolk by NMR

作　　者：邓冬艳 邓鹏翅[2] 宋红杰[1] 齐悦[1] Deng Dongyan;Deng Pengchi;Song Hongjie;Qi Yue(College of Chemistry,Sichuan University,Chengdu 610064,China;Analytical and Testing Center,Sichuan University,Chengdu 610064,China)

机构地区：[1]四川大学化学学院,四川成都610064 [2]四川大学分析测试中心,四川成都610064

出　　处：《实验技术与管理》2018年第10期50-53,共4页Experimental Technology and Management

基　　金：国家自然科学基金项目(21505095);四川大学实验技术立项项目(20170044)

摘　　要：以新鲜蛋液为原料,以磷脂酰胆碱为研究对象,采用乙醇(萃取)-丙酮(脱脂、重结晶)联合提取法提取、纯化蛋黄卵磷脂;利用核磁共振技术,以磷酸三苯酯为内标物,建立磷脂酰胆碱的定性定量分析方法。实验结果表明:料液比为1∶10、乙醇体积分数为95%、提取时间为60min时,所研究的几种蛋类蛋黄卵磷脂提取量为4.88~8.14mg/g;核磁共振氢谱、磷谱结果显示,这些蛋黄卵磷脂提取物中磷脂酰胆碱的百分含量为16.9%~27.4%,加标回收实验结果令人满意,表明该核磁共振方法能实现蛋黄中磷脂酰胆碱的有效分析测定。By using fresh egg liquid as the raw material and phosphatidylcholine as the research object,the ethanol(extraction)-acetone(degreasing,recrystallization)combined extraction method is used to extract and purify the yolk lecithin.The qualitative and quantitative analysis method for phosphatidylcholine is established by using the NMR technology with the triphenyl phosphate as internal standard.The experimental results show that when the ratio of the material to liquid is 1:10,the volume fraction of ethanol is 95%,the extraction time is 60 min,and the amount of the lecithin extracted from egg yolks is 4.88-8.14/g.The results of the NMR hydrogen spectrum and phosphorus spectrum show that the percentage of phosphatidylcholine in these yolk lecithin extracts ranges from 16.9%to 27.4%.The results of recovering the phosphatidylcholine from egg yolk by adding standard are satisfactory,which indicates that the method can be used for the determination of phosphatidylcholine in egg yolk.

关 键 词：禽蛋 磷脂酰胆碱 核磁共振 

分 类 号：TS253.2[轻工技术与工程—农产品加工及贮藏工程]