超高压液相色谱-质谱同时测定酿酒、制曲原料中多种真菌毒素的方法研究  

Simultaneous Detection of Multiple Mycotoxins in Liquor-Making/Starter-Making Raw Materials by UPLC-MS

作　　者：罗文业 卓毓崇 周艳 徐克赞 何世兴 时源 陈振 徐源 LUO Wenye;ZHUO Yuchong;ZHOU Yan;XU Kezan;HE Shixing;SHI Yuan;CHEN Zhen;XU Yuan(Langjiu Distillery Co.Ltd.,Gulin,Sichuan 646523,China)

机构地区：[1]四川省古蔺郎酒厂有限公司,四川古蔺646523

出　　处：《酿酒科技》2018年第10期24-27,共4页Liquor-Making Science & Technology

基　　金：四川省科技支撑计划专项项目(2016GZ0366);四川省自主创新成果产业化专项项目(2016LZ50232)

摘　　要：采用超高压液相色谱-质谱法同时检测酿酒、制曲原料中黄曲霉毒素B1、脱氧雪腐镰刀菌烯醇、赭曲霉毒素A、玉米赤霉烯酮4种真菌毒素,同时采用梯度洗脱,在质谱正离子模式下监测并完成检测,检出限可满足国家标准《食品安全国家标准食品中真菌毒素限量》(GB 2761—2017)对粮食中4种真菌毒素的要求。该方法灵敏度高、准确性好,而且简单快速,可用于粮食中真菌毒素的检测及其风险评估。Four kinds of mycotoxins including aflatoxin B1,deoxynivalenol,ochratoxin A,and zearalenone in liquor-making/startermaking raw materials were detected simultaneously by UPLC-MS.Meanwhile,gradient elution was adopted and the detection was finished under mass spectrometry positive ion mode.The detection limits met the requirements in national standard GB 2761-2017.This method had the advantages including high sensitivity,high accuracy and simple and fast operation,and it could be used in the detection of mycotoxins in food and the related risk evaluation.(Trans.by YUE Yang)

关 键 词：超高压液相色谱-质谱 测定 真菌毒素 方法 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.2[轻工技术与工程—食品科学与工程]