川芎、当归双提法提取工艺及挥发油包合工艺研究  

Study on Ambi-extracting and Inclusion Process of Volatile Oil from Chuanxiong Rhizoma and Angelicae Sinensis Radix

作　　者：王美玲[1] 倪健[1] 郭琳 陈功森[1] 于颖超 游龙泰 张海英 尹兴斌[1] 曲昌海[1] WANG Mei-ling;NI Jian;GUO Lin;CHEN Gong-sen;YU Ying-chao;YOU Long-tai;ZHANG Hai-ying;YIN Xing-bin;QU Chang-hai(College of Pharmacy,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中国中医药信息杂志》2018年第11期68-72,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市科技计划"十病十药"研发(Z171100001717023)

摘　　要：目的优选川芎、当归双提法提取及挥发油包合的最佳工艺。方法以挥发油提取量、水提液中阿魏酸提取量为指标,采用单因素试验考察提取工艺;以挥发油包合率及包合物得率为评价指标,采用饱和水溶液法,进行L9(34)正交试验,优选挥发油的包合工艺。结果川芎、当归的最佳提取工艺为:饮片不浸泡,加8倍量水,提取8 h。提取工艺验证试验显示,挥发油平均提取量为1.23 m L,水提液中阿魏酸平均提取量为0.387 9 mg/g。最佳包合工艺为:油(m L)∶β-环糊精(g)=1∶8,温度40℃,包合3 h。包合工艺验证试验显示,挥发油包合率、包合物得率平均值分别为74.89%、72.81%。结论本试验优选的川芎、当归双提法提取工艺及挥发油包合工艺稳定可行,可为制剂生产提供一定依据。Objective To optimize ambi-extracting and inclusion process of volatile oil from Chuanxiong Rhizoma and Angelicae Sinensis Radix.Methods With yield ratio of volatile oil and ferulic acid content in water extract as evaluation indexes,single factor experiments were used to study the extraction process.With the inclusion rate of volatile oil and yield of inclusion as evaluation indexes,saturated aqueous solution was used to L9(34)orthogonal experiments to reach optimum inclusion process.Results The optimum extraction process of Chuanxiong Rhizoma and Angelicae Sinensis Radix was extracted for 8 hours with 8 folds the amount of water,and without soaking.The validation experiments of extraction of volatile oil and ferulic acid content in water extract were 1.23 mL and 0.387 9 mg/g.The optimum conditions of inclusion process were as follows:volatile oil(mL):β-CD(g)was 1:8;inclusion temperature was 40℃;inclusion time was 3 hours.The validation experiments of inclusion rate of volatile oil and yield of inclusion were 74.89%and 72.81%.Conclusion Optimum ambi-extracting and inclusion process of volatile oil from Chuanxiong Rhizoma and Angelicae Sinensis Radix are feasible and stable,witch can provide certain supporting data for preparation production.

关 键 词：川芎 当归 阿魏酸 双提法 Β-环糊精 包合工艺 

分 类 号：R283.5[医药卫生—中药学]