超临界CO_2萃取淫羊藿中总多酚工艺的优化及其抗氧化活性  

Total polyphenols from Epimedium brevicornu,their supercritical CO_2 extraction technique optimization and the antioxidant activity

作　　者：王文静[1] 董泰玮 WANG Wen-jing;DONG Tai-wei(College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China;College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046 [2]成都中医药大学药学院,四川成都611137

出　　处：《中成药》2018年第11期2426-2430,共5页Chinese Traditional Patent Medicine

摘　　要：目的优化超临界CO_2萃取淫羊藿中总多酚工艺,并评价其抗氧化活性。方法在单因素试验基础上,以温度、压力、CO_2体积流量、时间为影响因素,总多酚萃取量为评价指标,Box-Behnken设计优化萃取工艺。再测定总多酚对DPPH自由基的清除作用。结果最佳条件为温度41. 6℃,压力30. 5 MPa,CO_2体积流量26. 3 L/h,时间2. 09 h,总多酚萃取量53. 55 mg/g。250μg/m L总多酚对DPPH的清除率可达81. 26%, IC50为112. 06μg/m L。结论该方法稳定可靠,可用于超临界CO_2萃取淫羊藿中抗氧化活性较强的总多酚。AIM To optimize the supercritical CO2 extraction technique for total polyphenols from Epirmeium brevicornu Maxim,and to evaluate the antioxidant activity.METHODS Witli temperature,pressure,CO2 volume flow rate and time as influencing factors,total polyphenols extraction content as an evaluation index,Box-Be-hnken design was applied to optimizing the extraction on the basis of single factor experiment.Subsequently the scavenging effect of total polyphenols on DPPH free radical was determined.RESULTS The optimal conditions were determined to be 41.6 d for temperature,30 5 MPa for pressure,26.3 Mh for CO2 volume flowrate,and 2.09 h for time,the total polyphenols extraction content was 53.55 mg/g.The scavenging rate of total polyphenols(250ug/mL)on DPPH free radical was 81.26%witii the IC50 of 112.06ug/mL.CONCLUSION Thi stableand reliable method can be used for the supercritical CO2 extraction for total polyphenols with strong antioxidant activity from E.brevicornu.

关 键 词：淫羊藿 总多酚 超临界CO2萃取 抗氧化活性 BOX-BEHNKEN设计 

分 类 号：R284.2[医药卫生—中药学]