艾纳香中的黄酮类化合物及其抗氧化与α-葡萄糖苷酶抑制活性研究  

Study on the Flavonoids in Blumea balsamifera DC. and Their Antioxidant Activity as well as α-Glucosidase Inhibitory Activity

作　　者：胡永 李亚男 李霞[3] 郝小江 杨万霞 顾玮[1,2] HU Yong;LI Ya-nan;LI Xia;HAO Xiao-jiang;YANG Wan-xia;GU Wei(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China;Guizhou Hongyu Pharmaceutical Co.,Ltd.,Guiyang 550018,China)

机构地区：[1]贵州医科大学省部共建药用植物功效与利用国家重点实验室 [2]贵州省中国科学院天然产物化学重点实验室,贵阳550014 [3]贵州宏宇药业有限公司,贵阳550018

出　　处：《天然产物研究与开发》2018年第11期1898-1903,共6页Natural Product Research and Development

基　　金：贵州省科技计划(黔科合支撑[2018]2799号);国家自然科学基金(31600280);贵州省自然科学基金(黔科合J字[2015]2105号);黔科合平台人才2017-5737

摘　　要：本文对艾纳香Blumea balsamifera DC.叶中的黄酮类成分及其抗氧化与α-葡萄糖苷酶抑制活性进行了研究。从艾纳香叶中分离得到9个黄酮类化合物,分别鉴定为:4'-甲氧基二氢槲皮素(1),柽柳黄素(2),3,3'-二甲氧基槲皮素(3),7,4'-二甲氧基二氢槲皮素(4),(2α,3β)-二氢鼠李素(5),艾纳香素(6),sterubin(7),enodicytol(8),二氢槲皮素(9)。化合物3、5、8为首次从艾纳香中分离得到。分别用DPPH法和PNPG法筛选化合物的抗氧化活性及α-葡萄糖苷酶抑制活性。结果表明化合物2、5~9表现出强抗氧化活性;化合物1~3、5、9表现有较高的α-葡萄糖苷酶抑制活性。该结果为艾纳香的品质评价和深度开发利用提供了理论依据。The study was focus on the flavonoids of Blumea balsamifera DC.and their bioactivities.The compounds of B.balsamifera were isolated and purified with various chromatographic techniques,nine flavonoids were isolated from the leaves of B.balsamifera,their structures were identified as dihydroquercetin-4′-methyl ether(1),tamarixetin(2),3,3′-di-O-methylquercetin(3),dihydroquercetin-7,4′-dimethyl ether(4),(2α,3β)-dihydrorhamnetin(5),blumeatin(6),sterubin(7),enodicytol(8),dihydroquercetin(9),respectively.Compounds 3,5 and 8 were isolated from this plant for the first time.DPPH radical scavenging method was used to evaluate antioxidant activity,and PNPG method was used to determineα-glucosidase inhibitory activity of these compounds.The results indicated that compound 2 and 5-9 showed strong antioxidant activities;and compounds 1-3,5 and 9 displayed stronger inhibitory activities than the positive control of acarbose.The results provided a theoretical basis for the quality evaluation and deep development and utilization of B.balsamifera.

关 键 词：艾纳香 黄酮 抗氧化活性 α-葡萄糖苷酶抑制活性 

分 类 号：R914.4[医药卫生—药物化学]