The expression characteristics of vitellogenin(VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis  

作　　者：LI Wanjuan XUE Shenglun PANG Min YUE Zonghao YANG Dazuo ZHOU Yibing ZHAO Huan 李婉娟;薛圣伦;庞敏;岳宗豪;杨大佐;周一兵;赵欢

机构地区：[1]Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province,Dalian Ocean University [2]Key Laboratory of Marine Ecology and Environmental Science and Engineering,First Institute of Oceanography,State Oceanic Administration

出　　处：《Journal of Oceanology and Limnology》2018年第6期2297-2307,共11页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.41306138);the National Marine Public Welfare Research Project(Nos.201305002,201305043);the Foundation of Education Department of Liaoning Province(No.L201609)

摘　　要：In order to investigate the endocrine toxicity of B(a)p to marine polychaete P erinereis aibuhitensis, vitellogenin(VTG) cDNA from the P. aibuhitensis was isolated, recombinated and expressed for the first time. The full length P. aibuhitensis vitellogenin gene(PaVTG) was 5 325 bp, and encoded 1 692 amino acids. It contained the vitellogenin_N domain of unknown function(DUF1943), a von Willebrand factor type D domain, as well as a conserved KALGNAG motif. The expression of VTG gene and protein were mainly up-regulated after exposed to B(a)p at transcriptional and translational levels. PaVTG gene expression did not change significantly at day 4. At day 7 PaVTG expression was up-regulated in 0.5 μg/L and 5 μg/L B(a)p group. At day 14 PaVTG was significantly up-regulated in 0.5–10 μg/L B(a)p. The protein expression of PaVTG in 0.5 μg/L and 10 μg/L B(a)p group was up-regulated with time prolonging, but the expression in 5 μg/L and 50 μg/L B(a)p group exhibited first increased and then decreased trend. With the increasing of B(a)p concentration PaVTG mRNA and protein expression both firstly increased then decreased. In contrast to B(a)p exposure, estradiol did not induce PaVTG gene and protein expression, until late times of exposure(14 d). Overall, the results in this study indicate that PaVTG could be used as a potential indicator of the effects environmental estrogenic compounds.In order to investigate the endocrine toxicity of B(a)p to marine polychaete P erinereis aibuhitensis, vitellogenin(VTG) cDNA from the P. aibuhitensis was isolated, recombinated and expressed for the first time. The full length P. aibuhitensis vitellogenin gene(PaVTG) was 5 325 bp, and encoded 1 692 amino acids. It contained the vitellogenin_N domain of unknown function(DUF1943), a von Willebrand factor type D domain, as well as a conserved KALGNAG motif. The expression of VTG gene and protein were mainly up-regulated after exposed to B(a)p at transcriptional and translational levels. PaVTG gene expression did not change significantly at day 4. At day 7 PaVTG expression was up-regulated in 0.5 μg/L and 5 μg/L B(a)p group. At day 14 PaVTG was significantly up-regulated in 0.5–10 μg/L B(a)p. The protein expression of PaVTG in 0.5 μg/L and 10 μg/L B(a)p group was up-regulated with time prolonging, but the expression in 5 μg/L and 50 μg/L B(a)p group exhibited first increased and then decreased trend. With the increasing of B(a)p concentration PaVTG mRNA and protein expression both firstly increased then decreased. In contrast to B(a)p exposure, estradiol did not induce PaVTG gene and protein expression, until late times of exposure(14 d). Overall, the results in this study indicate that PaVTG could be used as a potential indicator of the effects environmental estrogenic compounds.

关 键 词：Perinereis aibuhitensis VITELLOGENIN B(A)P EXPRESSION profiles 

分 类 号：P[天文地球]