正常免疫鼠人原代卵巢癌模型的建立  

Establishment of human ovarian cancer model in normal immune mice

作　　者：黄蓉 洪丰[2] 毕研贞 王云飞[4] 舒振锋 石秀玲[6] 杨飞 王卫 彭存旭[4] Huang Rong;Hong Feng;Bi Yanzhen(Jining Medical University Clinical Medica College,Jining 272000;The Affiliated Hospital of Jining Medical University,Jining 272000;Beijing You'an Hospital,Capital Medical University,Beijing 100000)

机构地区：[1]济宁医学院临床医学院,济宁272000 [2]济宁医学院附属医院肝病研究所,济宁272000 [3]首都医科大学附属北京佑安医院,北京100000 [4]济宁医学院附属医院妇科,济宁272000 [5]上海美峰生物技术有限公司,上海200120 [6]济宁医学院附属医院造口门诊,济宁272000 [7]济宁医学院附属医院病理科,济宁272000

出　　处：《现代妇产科进展》2018年第12期901-904,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金(No:81170395;81570556;81502255)

摘　　要：目的:正常免疫小鼠皮下接种人原代卵巢癌细胞与微载体(microcarrier 6)复合体,建立正常免疫鼠人原代卵巢癌模型。方法:将36只C57BL/6雌鼠按是否将人原代卵巢癌细胞与微载体共同培养随机分为原代卵巢癌细胞组、空载体组和三维原代卵巢癌细胞组。将人原代卵巢癌细胞与microcarrier 6共同培养,于小鼠皮下注射,观察小鼠的成瘤时间、成瘤率和病理学特点。结果:原代卵巢癌细胞组和空载体组均未成瘤,三维原代卵巢细胞组总成瘤率达58%,成瘤快,镜下观察符合人卵巢癌特点。结论:本实验成功建立了正常免疫小鼠人原代卵巢癌模型,该模型能更好地模拟卵巢癌在人体内的发生发展情况,可用于更多抗肿瘤药物研究。Objective:Human ovarian cancer cells were co-cultured with microcarrier 6 and inoculated subcutaneously into normal immunized mice in order to establish human immunized ovarian cancer cells.Methods:Thirty-six female C57BL/6 mice were randomly divided into primary ovarian cancer cells,empty carrier group and three-dimensional primary ovarian cells according to whether or not human ovarian cancer cells were inoculated into scaffold materials.After the human ovarian cancer cells were co-cultured with microcarrier 6,the mice were inoculated by subcutaneous injection.The time of tumor formation,the rate of tumor formation,and the pathological features of the mice were observed.Results:Both the primary ovarian cancer cells and the control group did not become tumors.The three-dimensional primary ovarian cells had total tumor formation rate of 58%.The tumor formation time was early.HE and immunohistochemical staining of the transplanted tumors were all consistent with the characteristics of human ovarian cancer.Conclusion:A human ovarian cancer model in normal immune mice was successfully established.The model can better simulate the occurrence and development of ovarian cancer in human body,and can also be used for more anti-tumor drug research.

关 键 词：卵巢癌 MICROCARRIER 动物模型 皮下移植瘤 

分 类 号：R737.33[医药卫生—肿瘤]