外切型蛋白酶法制备高档医用骨明胶工艺研究  

Study on extraction of high-grade medical bone gelatin by circumscribed protease

作　　者：张海林 朱友双 张玉然 仇艳新 吴红 杨志茹 ZHANG Hailin;ZHU Youshuang;ZHANG Yuran;QIU Yanxin;WU Hong;YANG Zhiru(College of Biological Sciences,Jining Medical University,Rizhao 276826,China)

机构地区：[1]济宁医学院生物科学学院,日照276826

出　　处：《济宁医学院学报》2018年第6期381-383,387,共4页Journal of Jining Medical University

基　　金：山东省自然科学基金博士基金项目(ZR2017BC044);山东省中医药科技发展计划项目(2017-270);国家自然(社会)科学基金培育项目(600327002)

摘　　要：目的探讨外切型蛋白酶法制备骨明胶的可行性及其制备条件的优化。方法以骨明胶的黏度、冻力、提胶率为参考指标,分别对浸酸终点pH、复合蛋白酶配方、酶解时间等参数进行优化,确定骨明胶最佳制备条件。结果最佳条件下,浸酸终点pH 2. 0,风味蛋白酶与2709蛋白酶质量比8∶2,酶解时间20 h时,所获骨明胶平均冻力为337 Bloom·g,黏度为6. 52 m Pa·S,提胶率为15. 3%。结论该法酶解终点易判断,节省了制备时间,同时大幅提高明胶黏度及动力,可获得符合国家标准的高档医用骨明胶,为后续生物酶法制备高档骨明胶的工业化应用奠定基础。Objective To optimize extraction conditions for high-grade medical gelatin by circumscribed protease.Methods In order to obtain the optimal viscosity,gel strength and yield,the composition of protease,pH and enzymolysis time for gelatin preparation were optimized.Results Optimum variables were the weight ratio of 8∶2 between flavourzyme and 2709 protease,pH of 2.0 and enzymolysis time of 20 h.Under this condition,the average gel strength,viscosity and yield were 337 Bloom·g,6.52 mPa·S and 15.3%,respectively.Conclusion The end point of this protocol was easily identified.Moreover,the viscosity and gel strength were significantly improved.This protocol will lay the foundation for the industrial application.

关 键 词：骨明胶 优化 复合蛋白酶 提取 

分 类 号：Q63[生物学—生物物理学]