高效液相色谱法同时检测荔枝果肉中甲基硫菌灵及其代谢物残留量  

Simultaneous Determination of Thiophanate-methyl and Its Metabolite Residues in Pulp of Litchi by HPLC Method

作　　者：王思威[1] 刘艳萍[1] 王潇楠 徐能莉 孙海滨[1] WANG Si-wei;LIU Yan-ping;WANG Xiao-nan(Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou,Guangdong 510640)

机构地区：[1]广东省农业科学院植物保护研究所,广东省植物保护新技术重点试验室,广东广州510640

出　　处：《安徽农业科学》2018年第35期174-176,共3页Journal of Anhui Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0200206-Y);国家荔枝龙眼产业技术体系项目(CARS-32-16);广东省省级科技计划项目(2017A030303038)

摘　　要：[目的]建立甲基硫菌灵和多菌灵在荔枝果肉中的高效液相色谱(HPLC)检测分析方法。[方法]样品采用甲酸-乙腈作为提取溶剂、乙二胺N-丙基硅烷吸附剂(PSA)进行净化,高效液相色谱检测分析荔枝果肉基质中的甲基硫菌灵和多菌灵残留量。[结果]甲基硫菌灵和多菌灵在为0.01～1.00 mg/kg添加水平下的添加回收率分别为78.9%～97.6%、79.8%～94.6%,相对标准偏差分别为2.08%～3.95%、3.04%～5.27%;最低检出量均为0.1 ng,最小检出浓度均为0.01 mg/kg。[结论]该前处理方法简便、有机溶剂用量少,准确度、精密度等均符合农药残留检测分析要求。[Objective]The research aimed to establish an efficient method for determination of thiophanate-methyl and carbendazim residues in pulp of litchi by QuEChERS and high performance liquid chromatography(HPLC)technique.[Method]The sample was purified by formic acid-acetonitrile as extraction solvent and ethylenediamine N-propylsilane adsorbent(PSA).The residue of thiophanate-methyl and carbendazim in litchi pulp matrix was analyzed by HPLC.[Result]The average recoveries of thiophanate-methyl ranged from78.9%to97.6%with the relative standard deviation(RSD)of2.08%-3.95%,the average recoveries of carbendazim were79.8%-94.6%with the RSD of3.04%-5.27%.The limit of detection and the lowest detection limits of method was0.1ng and0.01mg/kg,respectively.[Conclusion]This method is convenient,less organic solvents.The accuracy and precision could meet the requirements for pesticide residue analysis.

关 键 词：甲基硫菌灵 多菌灵 荔枝果肉 高效液相色谱法 代谢物 残留量 

分 类 号：TS207.53[轻工技术与工程—食品科学]