响应面法优化玫瑰茄花色苷的提取工艺  

Optimization of Extraction Process of Anthocyanin from Roselle by Response Surface Methodology

作　　者：钱镭[1] 刘婷[1] 辛雪 姜涛[1] 胡淑敏 屈岩峰[1] 任德财[1] QIAN Lei;LIU Ting;XIN Xue;JIANG Tao;HU Shu-min;QU Yan-feng;REN De-cai(cDepartment of Food and Environmental,East University of Heilongjiang,Harbin 150066,Heilongjiang,China;Heilongjiang Longdan Dairy Industry Polytron Technologies Co.,Ltd.,Harbin 150086,Heilongjiang,China)

机构地区：[1]黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨150066 [2]黑龙江龙丹乳业科技股份有限公司,黑龙江哈尔滨150086

出　　处：《食品研究与开发》2019年第1期156-162,共7页Food Research and Development

基　　金：黑龙江东方学院横向课题(HDFHX160108)

摘　　要：以玫瑰茄干花萼为原料,采用乙醇溶剂来提取花色苷。在选取乙醇浓度、提取温度、料液比、提取时间进行单因素试验的基础上,利用Box-Behnken设计四因素三水平的响应面优化试验。结果表明:对花色苷含量影响最大的是乙醇浓度,其次是提取温度和提取时间,影响最小的是料液比。最终确定最佳提取工艺参数为:乙醇浓度38%,提取温度66.6℃,料液比1∶24(g/mL),提取时间91 min,在此条件下玫瑰茄花色苷提取含量值达到5.285 mg/g。The anthocyanins were extracted from the roselle dried calyx with ethanol solvent.On the basis of single factor experiment of ethanol concentration,extraction temperature,material liquid ratio and extraction time,and a response surface optimization experiment with four factors and three levels was designed by Box-Behnken.The results showed that the most important factor affecting anthocyanin content was ethanol concentration,followed by extraction temperature and extraction time,and the smallest factor was the solidliquid ratio.Finally,the optimum extraction parameters were determined as follows:ethanol concentration was 38%,extraction temperature was 66.6℃,the solid-liquid ratio was 1:24(g/mL),and extraction time was 91 min.Under these conditions,the anthocyanin content from roselle was 5.285mg/g.

关 键 词：玫瑰茄 花色苷 提取 响应面分析法 

分 类 号：TS264.4[轻工技术与工程—发酵工程]