聚三亚甲基碳酸酯体外酶解性能的影响因素及其作用规律  

In vitro enzymatic degradation of poly(trimethylene carbonate): influencing factors and their effects

作　　者：李乌云塔娜 赵灵燕[1] 张巍 夏远[1] 杨立群 Li Wuyuntana;Zhao Lingyan;Zhang Wei;Xia Yuan;Yang Liqun(Inner Mongolia Medical University, Hohhot 010020, Inner Mongolia Autonomous Region, China;Liaoning Research Institute of Family Planning, China Medical University, Shenyang 110031, Liaoning Province,China)

机构地区：[1]内蒙古医科大学,内蒙古自治区呼和浩特市010020 [2]辽宁省计划生育科学研究院中国医科大学,辽宁省沈阳市110031

出　　处：《中国组织工程研究》2019年第6期945-950,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家十三五重点研发计划项目(2016YFC1000902);项目负责人:杨立群;国家自然科学基金项目(51503093);项目负责人:杨立群;辽宁省重点研发计划项目(2018225079);项目负责人:杨立群;辽宁省自然科学基金项目(20170540491);项目负责人:张巍;沈阳市高层次创新人才计划项目(RC170359);项目负责人:杨立群;沈阳市科学技术计划项目(F16-205-1-37);项目负责人:杨立群~~

摘　　要：背景:聚三亚甲基碳酸酯具有良好的生物相容性和生物降解性能,具有较大的临床应用前景。目前,国内外关于聚三亚甲基碳酸酯体外酶解性能影响因素及其作用规律的研究较少。目的:研究聚三亚甲基碳酸酯的体外酶解性能,并探讨影响聚三亚甲基碳酸酯体外酶解性能的因素及其作用规律。方法:通过开环聚合反应制备聚三亚甲基碳酸酯均聚物及其共聚物,2种均聚物的分子质量分别为135,256 kDa,共聚物的分子质量为238 kDa,将分子质量为256 kDa的均聚物制成棒条与膜片2种形状,其余2种样品制成棒条。将均聚物、共聚物样品分别置于脂肪酶溶液中,均聚物样品于第1,2,4,8,10,12周取出,共聚物样品于3,6,9,12,15 d后取出样品,测量样品质量变化,计算失重率及降解速率常数。结果与结论:(1)分子质量、形状及共聚改性对聚三亚甲基碳酸酯的体外酶解性能有显著影响;(2)当分子质量由135kD a增加至256kD a时,聚三亚甲基碳酸酯均聚物棒条的酶解速率常数由每周1.46%增加到每周3.81%,说明分子质量越高,聚三亚甲基碳酸酯均聚物的体外酶解速率越快;(3)当形状由棒条转换为膜片时,分子质量为256 kDa聚三亚甲基碳酸酯均聚物的酶解速率常数由每周3.81%增加到每周9.16%,说明膜片形状样品降解速率更快;(4)在分子质量为256 kDa聚三亚甲基碳酸酯结构中引入等摩尔比己内脂成分后,其酶解速率常数由每周3.81%增加到每周14.49%,说明在结构中引入聚己内酯成分,可加速聚三亚甲基碳酸酯的降解速率;(5)各因素对聚三亚甲基碳酸酯体外酶解速率的影响程度顺序为:共聚组成>样品形状>分子质量。BACKGROUND:Poly(trimethylene carbonate)has great potential in clinical applications due to the excellent biocompatibility and biodegradability.Little is reported on the factors influencing the in vitro enzymatic degradation of poly(trimethylene carbonate)and the underlying mechanism.OBJECTIVE:To investigate the in vitro enzymatic degradation of poly(trimethylene carbonate),and to explore the influencing factors and their effects on the poly(trimethylene carbonate)degradation.METHODS:poly(trimethylene carbonate)homopolymers and copolymers were prepared by ring-opening polymerization.The molecular mass of the two homopolymers was135and256kDa,respectively.The molecular mass of the copolymers was238kDa.The homopolymer of256kDa was made into two shapes(rods and films),and the other two samples were shaped into rods.The in vitro enzymatic degradation of poly(trimethylene carbonate)was conducted in lipase solutions,the homopolymer samples were taken out at1,2,4,8,10,and12weeks,and the copolymer samples were taken at3,6,9,12,and15days.The mass loss and degradation rate constant were measured.RESULTS AND CONCLUSION:The molecular mass and shape as well as molar ratio played important roles on the in vitro enzymatic degradation behavior of poly(trimethylene carbonate).As the molecular mass increased from135to256kDa,the degradation rate constant of poly(trimethylene carbonate)homopolymer increased from1.46%to3.81%,indicating that the higher the molecular mass,the higher degradation rate of poly(trimethylene carbonate).The poly(trimethylene carbonate)film presented with higher degradation rate than the cylinder one with the same molecular weight,and the degradation rate constant increased from3.81%to9.16%as the shape of poly(trimethylene carbonate)with a molecular weight of256kDa changed from rods to films.The introduction of polycaprolactone segment accelerated the degradation rate of poly(trimethylene carbonate).The degradation rate constant increased from3.81%to14.49%as the50mol%caprolactone content was introduced into the

关 键 词：聚三亚甲基碳酸酯 体外酶解 降解速率 开环聚合反应 键引发 键增长 键终止 材料试验 脂肪酶 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]