氨基葡萄糖盐酸盐及其衍生物对斑马鱼骨骼损伤修复的比较研究  

Comparison of the effect of glucosamine hydrochloride and its derivatives on the skeletal repair in zebrafish

作　　者：张文娟[1] 许璟瑾 姚丽云 李秀敏[1] 潘裕添[1] 薛钰[1,2] ZHANG Wenjuan;XU Jingjin;YAO Liyun;LI Xiumin;Pan Yutian;XUE Yu(Fujian Engineering Technology Research Center of Mushroom Industry, Minnan Normal University,Zhangzhou 363000, China;State Key Laboratory of Membrane Biology,Beijing 100101)

机构地区：[1]闽南师范大学福建省菌类活性物质工程技术研究中心,福建漳州363000 [2]膜生物学国家重点实验室,北京100101

出　　处：《中国比较医学杂志》2018年第12期9-13,31,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金青年基金(31501174);2017年福建省高校杰出青年科研人才培育计划项目;福建省自然科学基金(2015J05070);福建省教育厅科技项目(JA15306)

摘　　要：目的探究氨基葡萄糖盐酸盐(glucosamine hydrochloride,GAH)及其结构相似的衍生物对斑马鱼骨骼损伤的修复作用,并比较GAH与阿仑膦酸钠片对骨骼损伤的预防效果。方法将斑马鱼胚胎暴露在相同浓度下(0. 1%,1 mg/m L)的GAH,葡萄糖、甘露醇、半乳糖、N-乙酰葡萄糖胺溶液中,观察其对胚胎发育的影响。将枸橼酸铁铵(ferric ammonium citrate,FAC)诱导的骨质疏松症斑马鱼幼鱼暴露在0. 1%的GAH、葡萄糖、甘露醇、半乳糖和N-乙酰葡萄糖胺溶液中,利用茜素红、阿尔新蓝染色法观察骨骼的修复状况。同时,通过茜素红染色法比较GAH与阿仑膦酸钠片(alendronate sodium,AL)对骨质疏松的预防作用。结果相同浓度的不同药物对斑马鱼发育影响的大小依次为葡萄糖<甘露醇<GAH <N-乙酰葡萄糖胺<半乳糖;染色结果表明,与其他药物相比,GAH对斑马鱼骨骼损伤的修复有显著性差异,且对骨骼损伤的预防作用比AL更好。结论 GAH对斑马鱼骨骼损伤的修复有更好的治疗效果,且对骨质疏松有一定预防作用。同时本研究为斑马鱼模型在天然骨质疏松药物的筛选和产品开发的应用提供了研究基础和思路。Objective The aim of this study was to explore the reparative effects of glucosamine hydrochloride(GAH)and its derivatives on skeletal damage in zebrafish,and compare the preventive effects of GAH and alendronate sodium(AL),a drug known to be effective against osteoporosis.Methods Zebrafish embryos were exposed to various agents(GAH,glucose,mannitol,galactose,and acetylglucosamine)at the same concentration(0.1%,1mg/mL),and the mortality and phenotype were analyzed.Furthermore,a zebrafish model of osteoporosis induced by ferric ammonium citrate(FAC)was treated with the above agents to assess their reparative effects on the damaged skeleton examined using alizarin red and alcian blue staining.We also compared the preventive effects of GAH and AL on osteoporosis zebrafish.Results After treatment with GAH and its derivatives,the trend of their effects on embryonic development was glucose<mannitol<GAH<acetylglucosamine<galactose.Skeletal staining showed that GAH had the optimal reparative effect on FAC-induced osteoporosis in zebrafish and a better preventive effect than AL on FAC-induced skeletal damage.Conclusions Compared with other agents,GAH has an excellent reparative effect on zebrafish bone damage and a good preventive effect on osteoporosis in zebrafish.This study provides the foundation and approaches for screening of natural anti-osteoporosis drugs and product development using the zebrafish model.

关 键 词：氨基葡萄糖盐酸盐 糖类衍生物 骨骼修复 骨骼染色 

分 类 号：R-33[医药卫生]