转透明颤菌血红蛋白基因里氏木霉菌株固体发酵产纤维素酶的能力研究  

Study on the Ability of Producing Cellulase by Solid Fermentation of Transgenic Tremella Hemoglobin Gene Trichoderma reesei

作　　者：左婕 严琳[1] 王安 陈啟月 伍红[1] Zuo Jie;Yan Lin;Wang An(College of Life Science and Technology,Southwest University for Nationalities,Sichuan Chengdu 610041,China)

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《四川畜牧兽医》2018年第12期29-33,共5页Sichuan Animal & Veterinary Sciences

基　　金：四川省省级大学生创新创业训练计划项目(s201710656020)

摘　　要：本试验对转入透明颤菌血红蛋白基因的里氏木霉菌株(Tu6-VHb)与未转入该基因的里氏木霉菌株(Tu6)固体发酵产纤维素酶的能力进行了比较分析,并对Tu6-VHb菌株固体发酵产纤维素酶的培养条件进行了响应面优化研究。结果表明,Tu6-VHb菌株比Tu6菌株具有更强的产纤维素酶能力,其产纤维素酶活力提高了61%。通过响应面优化试验,获得Tu6-VHb菌株固体发酵产酶的最佳培养条件为:碳源配比(即麸皮与稻谷杆的比例)为2.89,营养液氮源配比(即尿素与硫酸铵的比例)为0.90,初始pH值为5.02。在此优化条件下进行固体发酵,每克培养基可以产纤维素酶123.81 U/g,与预测最大响应值129.61 U/g接近,且固体发酵产纤维素酶的活力较未优化前提高了3.08倍。The abilities of producing cellulase by solid fermentation of Trichoderma reesei strain which transferred the vitreoscilla hemoglobin gene(Tu6-VHb)and without vitreoscilla hemoglobin gene(Tu6)were compared in this paper,and the response surface optimization test was carried out on the condition of Tu6-VHb solid fermentation production of cellulase.The result showed that Tu6-VHb had stronger cellulase-producing ability than that of the Tu6,and its cellulase activity improved by61%.According to the response surface optimization test,the optimal culture conditions as follows:the ratio of carbon source(bran:rice straw)was2.89,the ratio of nitrogen source(urea:ammonium sulfate)was0.90,the initial value of pH was5.02.Solid fermentation was performed on this conditions,the cellulase production per gram of medium was123.81U/g,which was close to the predicted maximum response value(129.61U/g),and the activity of cellulase produced by solid fermentation increased by3.08times compared with that before optimization.

关 键 词：响应面 纤维素酶 固体发酵 转透明颤菌血红蛋白基因 里氏木霉菌 

分 类 号：Q933[生物学—微生物学]