13种河豚鱼COⅠ基因部分DNA序列分析及在物种鉴定中的应用  

Analysis of Partial DNA Sequence of COⅠGene and Its Application for Species Identification of Thirteen Species of Puffer Fish

作　　者：陈文炳[1] 缪婷玉[1] 翁国柱 陈融斌[3] 邵碧英[1] 彭娟[1] 江树勋[1] CHEN Wenbing;MIAO Tingyu;WENG Guozhu;CHEN Rongbin;SHAO Biying;PENG Juan;JIANG Shuxun(Inspection and Quarantine Technology Center, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China;Putian Entry-Exit Inspection and Quarantine Bureau, Putian 351100, China;Fisheries College, Jimei University, Xiamen 361021, China)

机构地区：[1]福建出入境检验检疫局检验检疫技术中心,福建福州350001 [2]莆田出入境检验检疫局,福建莆田351100 [3]集美大学水产学院,福建厦门361021

出　　处：《食品科学》2018年第24期145-148,共4页Food Science

基　　金：国家质量监督检验检疫总局科技计划项目(2013IK149).

摘　　要：应用COⅠ基因聚合酶链式反应(polymerase chain reaction,PCR)扩增通用引物,对福建省和海南省搜集的3属13种河豚鱼样品与9个未知种名的河豚鱼样品的COⅠ基因靶序列片段进行PCR扩增和测序,13个已知物种样品的DNA序列提交基因库(GenBank),取得相应的登录号。各物种COⅠ基因靶序列长度均为681 bp。应用DNAMAN V6软件对样品的DNA序列进行同源性比对分析,建立样品间系统关系同源树。基于COⅠ基因序列,供试13个样品被划分为4个类群组。群间的同源率为84%,群内同源率为90%～100%。根据序列同源性分析结果,9个未知种名的样品被归类到2个类群组中,判定这9个样品为东方鲀属或腹刺鲀属,其中1个样品(HNW2)为月腹刺鲀,4个样品(HNW3、HNW4、FJW2、FJW5)为棕斑腹刺鲀,1个样品(HNW1)为暗鳍腹刺鲀;3个样品(FJW1、FJW3、FJW4)为横纹东方鲀。探讨基于DNA条形码技术的COⅠ基因靶序列片段在河豚鱼种属鉴别中应用的可能性。Partial fragments of the cytochrome oxidase subunit I(CO I)genes from13species in3genera of puffer fish and9samples from unknown species collected in Fujian province and Hainan province were amplified by polymerase chain reaction(PCR)using universal primers and the PCR products were sequenced.The DNA sequences of13known species were submitted to the GenBank with accession numbers.The target gene sequence was681bp in length for all species.Sequence alignment was carried out using DNAMAN V6software,and a phylogenetic tree among species was established.Thirteen known species were divided into4groups,with84%homology between the groups and90%–100%within the groups.Nine samples of unknown puffer fish species were classed into2groups:the Takifugu and Gastrophysus genera;HNW2was identified as Gastrophysus lunaris,HNW3,HNW4,FJW2,and FJW5as G.spadiceus,HNW1as G.gloveri and FJW1,FJW3and FJW4as T.oblongus.

关 键 词：河豚鱼 CO I基因 DNA条形码 物种鉴定 

分 类 号：S917.4[农业科学—水产科学] Q78[生物学—分子生物学]