紫胶色酸A的荧光性质及其在检测中的应用  

Fluorescence Properties and Detection of Laccaic Acid A

作　　者：伊冠东 刘兰香[2] 雷福厚 张加研 李凯[2] 郑华[2] 张弘[2] 孙彦琳[1] YI Guandong;LIU Lanxiang;LEI Fuhou;ZHANG Jiayan;LI Kai;ZHENG Hua;ZHANG Hong;SUN Yanlin(Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, China;Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, China;Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi University for Nationalities, Nanning 530006, China;College of Chemistry and Engineering, Southwest Forestry University, Kunming 650224, China)

机构地区：[1]昆明理工大学化学工程学院,云南昆明650500 [2]中国林业科学研究院资源昆虫研究所,云南昆明650224 [3]广西民族大学广西林产化学与工程重点实验室,广西南宁530006 [4]西南林业大学化学工程学院,云南昆明650224

出　　处：《食品科学》2018年第24期311-316,共6页Food Science

基　　金：中央级公益性科研院所基本科研业务费专项(CAFYBB2017MA012);广西林产化学与工程重点实验开放课题(GXFC16-08)

摘　　要：考察紫胶色酸A的荧光性质和影响因素,探索其对金属离子的识别作用以及在实际检测中的应用。结果表明,溶剂的化学特性和极性、其自身浓度以及溶液的pH值都会影响紫胶色酸A的荧光强度和发射波长;Cu^(2+)、Fe^(3+)、Fe^(2+)和Pb^(2+)使紫胶色酸A的荧光猝灭,而Zn^(2+)和Al^(3+)使其荧光增强,其中Al^(3+)体现出显著增强紫胶色酸A荧光强度的特异性,其他金属离子对其荧光强度几乎没有影响。在Al^(3+)(0.04 mol/L)存在的条件下,紫胶色酸A的荧光强度与其浓度呈良好的线性关系。应用实验结果表明,以Al^(3+)为标准液,用荧光法检测两种不同饮料中添加的紫胶色酸A的含量,样品回收率在95.0%～109.3%之间,相对标准偏差在1.9%～5.4%之间。本实验开发了一种新的检测紫胶色酸A的方法,该方法具有实际应用价值。In this paper,the fluorescence properties of laccaic acid A and the factors affecting them were investigated.In order to develop a new method to detect laccaic acid A,its metal ion recognition capacity was evaluated.The results showed that the chemical properties and polarity of the solvent used to laccaic acid A,concentration and pH on affected the fluorescence intensity and emission wavelength of laccaic acid A.Cu^2+,Fe^3+,Fe^2+,and Pb^2+quenched the fluorescence intensity of laccaic acid A,while Zn^2+and Al^3+led to fluorescence enhancement.Notably,Al^3+showed significant specificity.Other metal ions had little effect on the fluorescence intensity.In the presence of Al^3+(0.04mol/L),the fluorescence intensity of laccaic acid A was linear with its concentration.The application results showed that laccaic acid A could be detected in two different beverages by a fluorescence method with Al^3+as the standard solution.The recovery rates of the method were between95.0%and109.3%,with relative standard deviations(RSDs)in the range of1.9%to5.4%.This new method has great potential for practical application.

关 键 词：紫胶色酸A 荧光 金属离子 检测方法 

分 类 号：TS202[轻工技术与工程—食品科学] TQ619.8[轻工技术与工程—食品科学与工程]