准噶尔山楂残渣多糖对D-半乳糖衰老模型小鼠的影响  

Effects of Polysaccharides from Crataegus songorica K. Koch. Fruits on D-Galactose-Induced Aging Mice

作　　者：马雪 吕海英 李进[1,2] 张玉 张俊[3] MA Xue;Lü Haiying;LI Jin;ZHANG Yu;ZHANG Jun(Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, ürümqi 830054, China;College of Life Science, Xinjiang Normal University, ürümqi 830054, China;Key Laboratory of Plant Stress Biology in Arid Land, ürümqi 830054, China)

机构地区：[1]新疆特殊环境物种保护与调控生物学实验室,新疆乌鲁木齐830054 [2]新疆师范大学生命科学学院,新疆乌鲁木齐830054 [3]干旱区植物逆境生物学重点实验室,新疆乌鲁木齐830054

出　　处：《食品科学》2018年第23期155-160,共6页Food Science

基　　金：新疆自治区重点实验室项目(XJDX1414-2015-03);国家自然科学基金青年科学基金项目(31200300);国家自然科学基金地区科学基金项目(31560058);自治区教育厅普通本科高等学校重点实验室项目(xjtswz-2016-08)

摘　　要：研究准噶尔山楂残渣中的多糖对D-半乳糖致衰老小鼠的影响。采用水提醇沉法从准噶尔山楂残渣中提取多糖,对D-半乳糖致衰老小鼠灌胃不同剂量(200、400、800 mg/kg)的多糖,测定小鼠肝、脑组织及血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、总抗氧化能力(total antioxidative capability,T-AOC)、丙二醛(malondialdehyde,MDA)含量,并观察肝、脑组织病理形态学变化。结果表明:与模型组相比,多糖高剂量组小鼠肝组织和血清中GSH-Px活力显著升高(P<0.05,P<0.01);脑组织和血清中T-AOC显著升高(P<0.01,P<0.05);肝、脑组织和血清中MDA含量显著降低(P<0.05,P<0.01)。多糖中、高剂量组小鼠肝索结构完整,肝细胞内无明显脂肪空泡;海马区神经元结构较为清晰,细胞排列整齐。因此准噶尔山楂多糖对D-半乳糖引起的小鼠衰老有一定抑制作用,这为新疆特有植物准噶尔山楂的高效利用提供了依据。This purpose of this study was to explore the effect of polysaccharides extracted from the residue left after the extraction of anthocyanins from Crataegus songorica K.Koch.fruits on D-galactose-induced aging mice.The polysaccharides were prepared by water extraction and alcohol precipitation and were orally administered to D-galactoseinduced aging mice at three different doses(200,400and800mg/kg).Glutathione peroxidase(GSH-Px)activity,total antioxidative capability(T-AOC)and malondialdehyde(MDA)content in brain,liver and serum were measured.Histological extraction of liver and brain tissues was carried out as well.The results showed that high-dose polysaccharide could significantly improve the activity of GSH-Px in liver and serum(P<0.05,P<0.01),increase T-AOC in brain and liver(P<0.01,P<0.05),decrease the content of MDA in liver,brain and serum(P<0.05,P<0.01)when compared with the model group.The structure of liver lobule was intact in mice from the medium-and high-dose polysaccharide group,with no apparent fat vacuoles being observed in hepatocytes.The structure of hippocampal nerve cells,arranged orderly,was clear.In conclusion,the polysaccharides from Crataegus songorica K.Koch.fruits can protect against D-galactose-induced aging in mice,which provides a clue for the efficient utilization of Crataegus songorica K.Koch..

关 键 词：准噶尔山楂 多糖 D-半乳糖 衰老 

分 类 号：R151.3[医药卫生—营养与食品卫生学]