南疆维吾尔族儿童龋病及16S rRNA高通量测序菌斑微生物群落结构  

Study on Caries and Microbial Community Structure by 16S rRNA High-throughput Sequencing in Uighur Children of Southern Xinjiang

作　　者：仵楠[1] 代海涛[1] 徐江[1] WU Nan;DAI Hai-tao;XU Jiang.(The First Affiliated Hospital, School of Medicine, Shihezi University. Shihezi 832008, China.)

机构地区：[1]石河子大学医学院第一附属医院口腔科,新疆石河子832008

出　　处：《口腔医学研究》2018年第12期1307-1311,共5页Journal of Oral Science Research

基　　金：2017年石河子大学医学院第一附属医院院级课题(编号:YB201703)

摘　　要：目的:分析南疆维吾尔民族儿童患龋情况及微生物学角度研究龋病可能高发的原因。方法:通过分层整群抽样随机抽取南疆第三师51团5个连队幼儿园3～5岁维吾尔族儿童296名调查患龋情况及充填率,应用Illumina Miseq高通量测序技术分析高龋儿童、无龋儿童口腔微生物群落结构及多样性。结果:南疆第三师51团维吾尔族3～5岁儿童总患龋率83.4%、龋均(3.07±3.22)、龋补充填率2.07%。5岁组儿童患龋率、龋均明显高于3岁组,不同性别儿童患龋率和龋均之间差异均无统计学意义。无龋组与高龋组口腔菌斑呈微生物多样性,共98个outs,归属于12个门、18个纲、25个目、34个科、60个属。无龋组微生物多样性较高龋组丰富,Streptococcaceae,Porphyromonadaceae、Fusobacteria、Veillonellaceae、peptostreptococcaceae在两组所有样本中均存在,在高龋组中构成比高于无龋组。结论:南疆维吾尔族儿童患龋率明显高于国内平均水平,应加强对南疆地区群众的口腔资源投入,当地维吾尔族儿童中高龋和无龋人群口腔微生物群落结构存在一定的差异,高龋组中优势菌(Streptococcaceae,Porphyromonadaceae、Fusobacteria、Veillonellaceae、peptostreptococcaceae)对龋病发生发展的作用还有待进一步的研究。Objective:To study the condition of dental caries in Uighur children in southern Xinjiang and analyze the reasons of high incidence of caries in microbiology.Methods:Caries and filling rate of296Uygur children aged3-5in5company kindergartens in southen xinjiang were randomly selected.The microbiological community structure and diversity of oral cavity in children were analyzed by illumina misequencing platform.Results:Tooth decay rate was83.4%,average caries was3.07±3.22,and supplementary filling rate was2.07%.The tooth decay rate and average caries of5year old group were higher than those of3year old group.There was no significant difference between the rate of caries and the average of caries in children with different gender.The dental plaque of two groups was various.There were98outs,belongs to12phylum,18classes,25orders,34families,and60genus.Streptococcaceae,porphyromonadaceae,fusobacteria,veillonellaceae,and peptostreptococcaceae were richer in high caries.Conclusion:The incidence of dental caries in Uygur children in southern Xinjiang is higher than the domestic average,and it is necessary to strengthen the input of oral resources in southern Xinjiang.There are some differences in the structure of oral microorganism community between caries and non-caries.The effects of streptococcaceae,porphyromonadaceae,fusobacteria,veillonellaceae,and peptostreptococcaceae on caries development need to be further studied.

关 键 词：龋病 菌斑 微生物群落结构 16S RRNA 高通量测序 

分 类 号：R788.1[医药卫生—口腔医学]