利用重组里氏木霉高效降解秸秆的研究  

Study on Straw Degradation Using Recombinant Trichoderma Reesei

作　　者：秦雪丽 夏颖[1] 赵杰[1] 夏黎明[1] QIN Xue-li;XIA Ying;ZHAO Jie;XIA Li-ming(Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical andBiological Engineering, Zhejiang University, Hangzhou 310027, China)

机构地区：[1]浙江大学生物质化工教育部重点实验室,浙江大学化学工程与生物工程学院,浙江杭州310027

出　　处：《高校化学工程学报》2018年第6期1374-1381,共8页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(21676247).

摘　　要：秸秆还田对于减少化肥使用量、改善土壤生态环境等具有重要作用。本文采用重组里氏木霉对秸秆进行快速降解研究,结果表明,重组里氏木霉在固态发酵中对湿度(60%～75%)、温度(24～32℃)的适应范围较宽,10%氨水常温预处理可加速秸秆的降解进程。重组里氏木霉在秸秆基质中可迅速生长并分泌纤维素酶、漆酶和木聚糖酶,其中滤纸酶活力(即纤维素酶总活力)第8天开始趋于平稳,最终活力为132.26 IU?g^(-1);内切型-β-葡聚糖酶(CMC酶)、漆酶和木聚糖酶活力在第10天达到峰值,分别为1313.15、8.11和5504.10 IU?g^(-1)。接种后第10天,秸秆纤维素、半纤维素和木质素的降解率分别为77.93%、55.12%和31.52%。重组里氏木霉的秸秆降解率比出发菌株及枯草芽孢杆菌分别提高了45.40%和76.37%。本文的研究结果对于进一步提高秸秆快速降解还田技术,改善生态环境具有重要意义。Straw is a useful biomass for reducing fertilizer usage and improving soil ecology.Recombinant Trichoderma reesei(T.reesei ZJ-09)was applied in this study for rapid degradation of straw.The results show that T.reesei ZJ-09can be used under a wide range of humidity(60%~75%)and temperature(24~32℃)during solid state fermentation(SSF),and straw decomposition can be accelerated after pretreatment with10%ammonia at room temperature.Furthermore,T.reesei ZJ-09grows rapidly in straw substrate and it secretes cellulase,xylanase and laccase during SSF.Profile of filter paper activity stabilizes at132.26IU·g-1on the8th day.The highest endo-β-glucanase(CMCase),laccase and xylanase activity are1313.15,8.11and5504.10IU·g-1,respectively on the10th day.The results show that the degradation rate of cellulose,hemicellulose and xylose are77.93%,55.12%and31.52%respectively at the10th day.T.reesei ZJ-09shows degradation rate45.40%higher than that of original strain and76.37%higher than that of Bacillus subtilis.The results are useful for further improvement of plant straw degradation.

关 键 词：重组里氏木霉 秸秆快速降解 纤维素酶 漆酶 木聚糖酶 

分 类 号：Q814[生物学—生物工程]