碱法破壁-酶法提取葡萄酒废酵母细胞壁多糖的工艺研究  

Extraction technology of polysaccharides from the cell wall of spent yeasts of wine by alkaline breaking-enzymatic extraction method

作　　者：石豪磊 赵国正 孔祥君 栾俊家 孔国光 牌延惠 张丽君 许维娜 SHI Haolei;ZHAO Guozheng;KONG Xiangjun;LUAN Junjia;KONG Guoguang;PAI Yanhui;ZHANG Lijun;XU Weina(School of Pharmacy (School of Enology), Binzhou Medical University, Yantai 264003, China;Shandong Provincial Key Laboratory of Wine Microbial Fermentation Technology, Yantai Zhangyu Group Co., Ltd., Yantai 264001, China;MabPlex International Biomedical Co., Ltd., Yantai 264006, China)

机构地区：[1]滨州医学院药学院(葡萄酒学院),山东烟台264003 [2]烟台迈百瑞国际生物医药有限公司,山东烟台264006 [3]烟台张裕集团有限公司山东省葡萄酒微生物发酵技术重点实验室,山东烟台264001

出　　处：《中国酿造》2018年第12期82-86,共5页China Brewing

基　　金：国家级大学生创新创业训练计划项目(201710440156);山东省自然科学基金(ZR2017LC020;ZR2015HL040);滨州医学院科研启动基金(BY2015KYQD05)

摘　　要：以工业发酵产生的葡萄酒废酵母泥为材料,过筛法除去葡萄果皮、果籽等杂质后,以细胞破壁液蛋白质含量、多糖提取率为评价指标,通过单因素试验和正交试验对碱法破壁-酶法提取细胞壁多糖工艺进行优化,以期提高多糖提取率。结果表明,最佳工艺条件为细胞破壁碱(KOH)处理温度为60℃,碱(KOH)质量浓度为70 g/L,处理时间为1.5 h,80 kHz超声辅助;酶法提取多糖最佳工艺为酶作用温度50℃,酶解时间1.5 h,中性蛋白酶添加量0.3%,初始p H值7.0。在此优化条件下,葡萄酒废酵母多糖提取率为21.08%。Using the spent yeast mud produced by industrial fermentation as material,after removing impurities such as grape peel and fruit seeds by sieving method,and using protein concentration of cell wall breaking liquid and the extraction rate of polysaccharides as evaluation indexes,the alkali breaking and enzymatic extraction technology were optimized through single factor experiments and orthogonal experiments,in order to obtain high yield of polysaccharides.The results showed that the optimum technological conditions were as follows:alkali(KOH)concentration of cell wall breaking70g/L,treatment temperature60℃and time1.5h assisted by ultrasonic treatment of80kHz.The optimal enzymatic extraction technique condition was enzymatic temperature50℃,hydrolysis time1.5h,neutral protease addition0.3%,and initial pH value7.0.Under the optimal conditions,the final polysaccharides extraction rate of spent yeast was21.08%.

关 键 词：细胞壁多糖 碱法破壁 酶法提取 工艺优化 

分 类 号：R284.2[医药卫生—中药学]