通过式高效净化-超高效液相色谱-串联质谱测定猪肉、猪肝及其脂肪组织中21种三唑类杀菌剂  

Detection of Twenty-one Triazole Fungicides in Pork,Pork Liver and Pork Fat by Going Through Column Purification and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：李晓丹 汪建妹[2] 王向军[1] 杨华[2] 吉小凤[2] 徐杰[2] 钱鸣蓉[2] LI Xiao-Dan;WANG Jian-Mei;WANG Xiang-Jun;YANG Hua;JI Xiao-Feng;XU Jie;QIAN Ming-Rong(College of Forestry and Bio-technology,Zhejiang Agricultural and Forestry University,Hangzhou 311300,China;Institute of Quality and Standard for Agro-products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江农林大学林业与生物技术学院,杭州311300 [2]浙江省农业科学院农产品质量标准研究所,杭州310021

出　　处：《分析化学》2019年第2期297-305,共9页Chinese Journal of Analytical Chemistry

基　　金：农业行业标准制定项目(No.2016R19A24C08);国家风险评估项目(No.GJFP2018007)资助~~

摘　　要：建立了猪肉、猪肝及其脂肪组织中21种三唑类杀菌剂的超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法。样品经乙腈提取,浓缩后加水混匀,经PRiME HLB柱净化,收集流出液,用UPLC-MS/MS法测定,基质标准曲线定量。21种三唑类杀菌剂在0.5～100μg/L浓度范围内线性良好,相关系数均大于0.99;在3个添加浓度(2.0、5.0和10. 0μg/kg)下,各杀菌剂的回收率为69. 5%～109. 5%,相对标准偏差为0. 4%～10.8%。此方法前处理操作简便,灵敏度高,精密度好,能较大程度除去基质干扰,可用于猪肉、猪肝及其脂肪组织中21种三唑类杀菌剂残留的测定。A ultra performance liquid chromatography-tandem mass spectrometry method for determination of triazole fungicides in pork,pork liver and pork fat tissue was established.Samples were extracted with acetonitrile,concentrated,and well-mixed with water.The mixed extract was then purified by a PRiME HLB column,and the elution was collected and detected by ultra performance liquid chromatography-tandem mass spectrometry.The matrix-matched standard curve was applied for quantification.The curve was linear in the range of 0.5-100μg/L with the correlation coefficients of higher than 0.99.The recoveries ranged from 69.5%to 109.5%at the spiked levels of 2.0μg/kg,5.0μg/kg and 10.0μg/kg,with relative standard deviations of 0.4%-10.8%.The method has many advantages such as simple operation,high sensitivity and good precision,and can largely eliminate the interference in the matrix and is suitable for the residue detection of 21 triazolecompounds in the muscle,liver and fat tissues of animal derived food.

关 键 词：超高效液相色谱-串联质谱 猪肉 猪肝 猪脂肪 三唑类杀菌剂 

分 类 号：O657.63[理学—分析化学] TS251.51[理学—化学]