高效液相色谱法测定大蒜愈伤组织中大蒜素的含量  

Determination of Allicin Content in Garlic Callus by High Performance Liquid Chromatography

作　　者：刘兰 陈雅楠 尚云涛 范宝莉[1,2] 王振英 LIU Lan;CHEN Ya-nan;SHANG Yun-tao;FAN Bao-li;WANG Zhen-ying(College of Life Science,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin 300387,China;Tianjin Key Laboratory of Water Resources and Water Environment,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津市动植物抗性重点实验室,天津300387 [3]天津市水资源与水环境重点实验室,天津300387

出　　处：《中国调味品》2019年第2期1-4,9,共5页China Condiment

基　　金：天津市企业科技特派员项目(18JCTPJC59100);天津市农委科技成果转化与推广项目(201302030);天津师范大学应用开发基金项目(52XK1505;043135202-XK1707)

摘　　要：建立了高效液相色谱法测定大蒜愈伤组织中大蒜素含量的分析方法。色谱柱为Eclipse Plus C18(4.6mm×250mm,5μm),柱温35℃,流动相为甲醇-水含0.2%甲酸(80∶20),流速1.0mL/min,二极管阵列检测器检测波长240nm,进样量50μL。结果显示:大蒜素在0.30～4.57μg/mL范围内线性关系良好,相关系数R2=0.999,加标回收率为95%～102%。该方法灵敏度高,重现性好,操作简便,适用于大蒜愈伤组织中大蒜素含量的检测。Develop a method for the determination of allicin in garlic callus by high performance liquid chromatography(HPLC).The conditions of HPLC are as follows:the chromatography column is Eclipse Plus C 18(4.6 mm×250 mm,5μm),the column temperature is 35℃,the mobile phase is methanol∶water(0.2%formic acid)of 80∶20(V/V),the flow rate is 1.0 mL/min,the DAD detector wavelength is 240 nm,and the sample injection volume is 50μL.The results show that the content of allicin has a good linear relationship in the range of 0.30~4.57μg/mL,the correlation coefficient is 0.999,and the average recovery of allicin is 95%~102%.This method is accurate,reliable,simple and efficient for the detection of allicin in garlic callus.

关 键 词：大蒜愈伤组织 大蒜素 高效液相色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学]