虫草素对MPTP诱导的帕金森病小鼠运动与认知能力的影响  

Effects of cordycepin on motor and cognition in Parkinson disease mice induced by MPTP

作　　者：刘丽 罗广轩 林健琳 关燕清[1] 刘文晓[1] 肖鹏[1] 李楚华[1] LIU Li;LUO Guang-xuan;LIN Jian-lin;GUAN Yan-qing;LIU Wen-xiao;XIAO Peng;LI Chu-hua(School of Life Science,South China Normal University,Guangzhou 510631,China)

机构地区：[1]华南师范大学生命科学学院,广东广州510631

出　　处：《中国病理生理杂志》2019年第2期326-331,共6页Chinese Journal of Pathophysiology

基　　金：广州市科技计划项目(No.201510010171);华南师范大学研究生创新计划项目

摘　　要：目的:探索虫草素(cordycepin)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)诱导的帕金森病小鼠运动与认知能力的影响。方法:将C57BL/6小鼠每天腹腔注射MPTP (30 mg/kg),连续8 d,以建立帕金森病小鼠模型。HE染色检测虫草素对MPTP诱导小鼠黑质致密部(sub-stantia nigra pars compacta,SNpc)细胞数目的影响;Western blot检测黑质(substantia nigra,SN)内酪氨酸羟化酶(tyrosine hydroxylase,TH)表达量的变化;采用旷场实验(open-filed test,OFT)、自发交替行为(spontaneous alterna-tion behavior,SAB)及水迷宫实验(water maze test,WMT)分别检测虫草素对帕金森病小鼠自发活动、情绪变化和认知行为的影响。结果:HE染色结果表明,虫草素显著抑制由MPTP引起的小鼠SNpc细胞数目减少(P <0.05);Western blot结果表明MPTP显著降低SN内TH的表达量(P <0.05),而虫草素可以逆转这种变化(P <0.05);虫草素可提高小鼠在OFT中的平均速度(P <0.05),并增加小鼠在SAB中的总进臂次数,提高正确率(P <0.05),但对WMT潜伏期并没有明显的影响。结论:虫草素能够减轻MPTP诱导的帕金森病小鼠早期的运动障碍和探索能力降低,但对其记忆障碍并没有明显的影响。AIM:To investigate the effects of cordycepin on the motor and cognition in Parkinson disease mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).METHODS:C57BL/6 mice were intraperitoneally injected with MPTP at a dose of 30 mg/kg daily for consecutive 8 d to establish the model of Parkinson disease.HE staining was used to observe the cell number in the substantia nigra pars compacta(SNpc)from the mice.Western blot was used to detect the protein level of tyrosine hydroxylase(TH)in substantia nigra(SN).The effects of cordycepin on the motor,emotional change and cognitive behavior of the Parkinson disease mice were examined by open-field test(OFT),sponta-neous alternating behavior(SAB)and water maze test(WMT),respectively.RESULTS:Cordycepin significantly reduced the apoptosis of cells in SNpc and reversed the decrease in the expression of TH in SN induced by MPTP(P<0.05).Furthermore,cordycepin was able to improve the average speed in OFT(P<0.05),and increased the total number of arm entry and the accuracy in SAB(P<0.05),but had no obvious effect on the latency in WMT.CONCLUSION:Cordycepin is capable of attenuating the impairments of motor and explorative ability in the early stage of Parkinson disease mice,but does not alter the cognitive dysfunction.

关 键 词：虫草素 帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 黑质致密部 酪氨酸羟化酶 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]