食用色素藻蓝蛋白对非小细胞肺癌A549细胞体外增殖和凋亡的影响  

Effect of C-Phycocyanin as a Food Pigment on Proliferation and Apoptosis of Non-small Cell Lung Cancer A549 Cells

作　　者：郝帅[1] 闫燕 李爽[1] 黄伟伟 王成涛[1] HAO Shuai;YAN Yan;LI Shuang;HUANG Weiwei;WANG Chengtao(Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology and Business University,Beijing 100048,China;Beijing Novogene Bioinformatics Technology Co.Ltd.,Beijing 100083,China)

机构地区：[1]北京食品营养与人类健康高精尖创新中心北京市食品添加剂工程技术研究中心北京工商大学,北京100048 [2]北京诺禾致源生物信息科技有限公司,北京100083

出　　处：《食品科学》2019年第3期158-164,共7页Food Science

基　　金：国家自然科学基金青年科学基金项目(31701575);国家自然科学基金面上项目(31571801);北京市优秀人才培养资助青年骨干个人项目(2016000020124G025);"十三五"时期北京市属高校高水平教师队伍建设支持计划项目(CIT&TCD201704042);"十三五"国家重点研发计划重点专项(2016YFD0400502-02);北京市科技计划项目(Z171100002217019);科技创新服务能力建设-科技成果转化-提升计划项目(PXM2016-014213-000034)

摘　　要：本研究以人类非小细胞肺癌A549细胞系为模型,采用藻蓝蛋白处理体外培养的细胞,分别从细胞存活率、细胞形态、细胞凋亡程度以及周期分布4个方面阐述了藻蓝蛋白对A549细胞的影响。结果表明,藻蓝蛋白能够显著降低A549细胞的存活率与生长速率(P<0.05),引起细胞形态发生非正常改变;同时上调促凋亡基因Bax、Bad,下调抑凋亡基因Bcl-2、Bcl-xl的表达而诱导细胞凋亡;此外,藻蓝蛋白能够异常调控细胞周期G1/S转换点相关基因的表达而引起细胞发生G1期阻滞。研究结果揭示了藻蓝蛋白对A549肺癌细胞具有显著的抑制作用,为开发新型抗癌类功能性食品着色剂提供了必要的理论依据。The objective of this study was to investigate the effect of C-phycocyanin(C-PC)on the survival rate,morphology,apoptosis and cell cycle distribution of non-small cell lung cancer A549 cells.The results showed that C-PC could significantly reduce the survival rate and growth rate of A549 cells(P<0.05).It also could induce apoptosis by up-regulating the mRNA and protein expressions of the Bax and Bad genes and down-regulating the mRNA and protein expressions of the Bcl-2 and Bcl-xl genes.In addition,C-PC could regulate the expression of G1/S check point-related genes,thereby causing G1 phase arrest.These results indicated C-PC had a significant inhibitory effect on A549 cells,which could provide the essential theoretical basis for the development of new anti-cancer food colorants.

关 键 词：藻蓝蛋白 A549细胞 凋亡 细胞周期 细胞生长 

分 类 号：TS202.3[轻工技术与工程—食品科学] R151.1[轻工技术与工程—食品科学与工程]