油菜蜂花粉及其蜂粮不同溶剂提取物抗氧化活性研究  

Study on Antioxidant Activity of Different Solvent Extracts of Rape Bee Pollen and Rape Bee Bread

作　　者：牛德芳[1] 王波 张静[2] 殷玲[1] 刘萍[1] 战旭梅[1] 陈玉勇[1] NIU De-fang;WANG Bo;ZHANG Jing;YIN Ling;LIU Ping;ZHAN Xu-mei;CHEN Yu-yong(Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,Jiangsu,China;Sericultural Institute of Heilongjiang Province,Harbin 150086,Heilongjiang,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]黑龙江省蚕业研究所,黑龙江哈尔滨150086

出　　处：《食品研究与开发》2019年第6期42-46,共5页Food Research and Development

基　　金：江苏农牧科技职业学院科研课题(NSF201506);2015年江苏省大学生创新创业训练计划项目(201512806015Y);2017年江苏省大学生创新创业训练计划项目(201712806020X)

摘　　要：以油菜蜂花粉及其同种植物源的蜂粮为原料,采用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS]自由基清除两种体外抗氧化方法,评价并比较油菜蜂花粉及其蜂粮水提物和醇提物的体外抗氧化活性。结果表明:4种提取物对DPPH自由基和ABTS+·均具有一定的清除能力,依次为蜂花粉醇提物、蜂粮醇提物>蜂粮水提物>蜂花粉水提物,其中蜂花粉及其蜂粮醇提物清除能力最强,IC50值分别为0.204 mg/mL～0.290 mg/mL和0.094 mg/mL～0.167 mg/mL,二者没有显著差异(p>0.05);2种醇提物的体外抗氧化活性与蜂粮水提物、蜂花粉水提物存在显著差异(p<0.05)。结果进一步证实蜂花粉及其蜂粮均具有一定的体外抗氧化活性,为其在抗氧化功能性食品等领域的开发应用奠定了理论基础。The in vitro antioxidant activities of rape bee pollen water extract,rape bee bread water extract,rape bee pollen ethanol extract and rape bee bread ethanol extract were evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging method and ABTS+·scavenging method.Results indicated that all the four kinds of extracts had scavenging effect on DPPH free radicals and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)free radicals,the strongest antioxidant activity was rape bee pollen ethanol extract and/or rape bee bread ethanol extract,followed by rape bee bread water extract and rape bee pollen water extract.Moreover,the scavenging activities of two kings of ethanol extracts against DPPH free radicals and ABTS^+·revealed no difference(p>0.05)and their IC50 values ranged from 0.204 mg/mL to 0.290 mg/mL and ranged from 0.094 mg/mL to 0.167 mg/mL,respectively.However,the antioxidant activities of two kings of ethanol extracts,rape bee bread water extract and rape bee pollen water extract displayed a significant difference(p<0.05).These results further confirmed that bee pollen and bee bread had antioxidant activities in vitro which laid the theoretical foundation for the development and application of antioxidant functional foods.

关 键 词：蜂花粉 蜂粮 抗氧化活性 1 1-二苯基-2-三硝基苯肼 2 2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]