淘米水发酵物中2种有机酸的测定  

Determination of organic acids in fermented rice-washing water

作　　者：杜刚[1] 陆小凯 刘仙花 洪耀强 杨海英 DU Gang;LU Xiao-kai;LIU Xian-hua;HONG Yao-qiang;YANG Hai-ying(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education,Kunming 650500,China;School of Chemistry and Environment,Yunnan Minzu University,Kunming 650500,China)

机构地区：[1]云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室,云南昆明650500 [2]云南民族大学化学与环境学院,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2019年第2期114-117,共4页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(81860624)

摘　　要：采用高效液相色谱法分析测定淘米水发酵液中的有机酸,确定了淘米水发酵液中有机酸分析测定的色谱条件,采用Agilent Zorbax SB-Aq(4.6 mm×150 mm,5μm)色谱柱,流动相为1%甲醇-99%NaH_2PO_4溶液(20 mmol/L,pH=2.0);紫外检测波长210 nm;流速0.4 mL/min;柱温25℃.结果表明,在该条件下淘米水发酵液中的有机酸得到了较好分离,分离检测出乳酸、乙酸2种有机酸.该分析方法简单准确,适用于淘米水发酵液中有机酸的测定分析.A HPLC(high performance liquid chromatography) method was developed for the determination of organic acids in fermented rice-washing water. HPLC separation was carried out in a Zorbax SB-Aq column(4.6 mm×150 mm,5 μm) with methanol -20 mmol/L NaH2PO4 solution (1∶99, V/V ) as the mobile phase. The velocity was 0.4 mL/min,the column temperature was 25 ℃,and the detection wave length was 210 nm. The results showed that the lactic acid and acetic acid in the fermented rice-washing water were separated and determined. This simple and accurate method could be used for the determination of organic acids in the fermented rice-washing water.

关 键 词：淘米水发酵液 乳酸 乙酸 高效液相色谱法 

分 类 号：O658[理学—分析化学]