环草石斛多酚成份及其生物活性  

Polyphenols from Dendrobium loddigesii and their biological activities

作　　者：李雪文 陈慧萍 何伟波 杨文铃 倪飞雨 黃子雯 胡海燕[1] 王军[1] LI Xuewen;CHEN Huiping;HE Weibo;YANG Wenling;NI Feiyu;HUANG Ziwen;HU Haiyan;WANG Jun(School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]中山大学药学院,广东广州510006

出　　处：《中山大学学报（自然科学版）》2019年第2期96-102,共7页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省科技计划项目(2014A020221006);广东省自然科学基金(2015A030313119)

摘　　要：为了研究环草石斛酚类成分及其生物活性,采用多种色谱技术对环草石斛丙酮(φ=80%)提取物进行了分离,所得化合物采用质谱、核磁等波谱技术进行了结构解析,并评价了各化合物的抗氧化、α-葡萄糖苷酶抑制及抗炎等活性。结果共分离鉴定了15个酚类物质,分别是chrysotoxol A(1)、neoechinulin A(2)、3,6,9-三羟基-3,4-二氢蒽-1(2H)-酮(3)、4,4′-二羟基-3,5-二甲氧基联苄(4)、柚皮素(5)、5,4′-二羟基-7,3′,5′-三甲氧基黄烷酮(6)、5,7,4′-三羟基-3′,5′-二甲氧基黄烷酮(7)、batatasin-III(8)、3,3′,5-三羟基联苄(9)、trigonopol B(10)、丁香脂素(11)、2,4,7-三羟基-9,10-二氢菲(12)、loddigesiinol B(13)、loddigesiinol I(14)、loddigesiinol J(15);其中化合物1-11为首次分自环草石斛的多酚。在所选实验条件下,化合物1-4、6、7、12的生物活性为首次评价;化合物1、3、12的强抗氧化活性,1、9的强α-葡萄糖苷酶抑制活性,1、12、14、15的强抗炎活性为首次报道。To study the polyphenols from Dendrobium loddigesii and their biological activities,the 80%acetone extract from D.loddigesii was isolated and purified by chromatography techniques;the structures of obtained compounds were identified with spectroscopic techniques;the antioxidative,α-glucosidase inhibitory and anti-inflammatory activities of compounds were evaluated.Fifteen compounds were isolated and identified as chrysotoxol A(1),neoechinulin A(2),3,6,9-trihydroxy-3,4-dihydroanthracen-1(2H)-one(3),4,4′-dihydroxyl-3,5-dimethoxylbibenzyl(4),naringenin(5),5,4′-dihydroxy-7,3′,5′-trimethoxyflavanone(6),5,7,4′-trihydroxy-3′,5′-dimethoxyflavanone(7),batatasin-III(8),3,3′,5-trihydroxybibenzyl(9),trigonopol B(10),syringaresinol(11),2,4,7-trihydroxyl-9,10-dihydrophenanthrene(12),loddigesiinol B(13),loddigesiinol I(14),loddigesiinol J(15),respectively.Compounds 1-11 among them were isolated from D.loddigesii for the first time.The biological activities of compounds 1-4,6,7 and 12 were evaluated for the first time under trial conditions.It was reported for the first time that compounds 1,3 and 12 had a strong antioxidant activity,compounds 1 and 9 showed a strongα-glucosidase inhibitory activity,and compounds 1,12,14 and 15 had a strong anti-inflammatory activity.

关 键 词：环草石斛 多酚 分离纯化 抗氧化活性 α-葡萄糖苷酶抑制活性 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]