PNU通过PI3K/AKT信号通路上调星形胶质细胞HSP70的表达  

PNU Upregulates HSP70 Expression via PI3K/AKT Signaling Pathway in Astrocytes

作　　者：董智慧[1,2] 吕菊 任真奎 谢鹏 胡玉梅 官志忠[1,2,4] 吴昌学[1,2] 禹文峰[1,2] DONG Zhihui;LV Ju;REN Zhenkuil;XIE Peng;HU Yumei;GUAN Zhizhong;WU Changxue;YU Wenfeng(Key Laboratory of Medical Molecular Biology,Guizhou Medical University,Guiyang 550004,Guizhou,China;Key Laboratory of Endemic and Ethnic Diseases,Ministry of Education,Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Clinical Laboratory,People's Hospital,Qianxinan Buyei and Miao Autonomous Prefecture,Xingyi 562400,Guizhou,China;Department of Pathology,Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学分子生物学重点实验室,贵州贵阳550004 [2]贵州医科大学地方病与少数民族疾病教育部重点实验室,贵州贵阳550004 [3]黔西南州人民医院检验科,贵州兴义562400 [4]贵州医科大学病理学教研室,贵州贵阳550004

出　　处：《贵州医科大学学报》2019年第3期254-258,263,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81360199);教育部科学技术研究项目(213032A);贵州省国际科技合作计划项目[黔科合外G字(2013)7026号];贵州省创新计划项目[黔教合协同创新中心(2014)06];贵州省教育厅项目[黔教合外G字(2013)63]

摘　　要：目的:研究PNU对星形胶质细胞热休克蛋白70(HSF70)表达的影响,并探讨其机制。方法:分离新出生24 h内乳鼠大脑星形胶质细胞原代培养并鉴定,传1～3代待细胞成熟后分为正常对照组、PNU处理组,PI3K/AKT信号通路阻断剂(LY294002)处理组和PNU+LY294002处理组,正常对照组不做任何处理,PNU处理组用5μmol/L PNU分别处理6、12、18、24 h,LY294002处理组只加10μmol/L LY294002处理,PNU+LY294002处理组是先加10μmol/L LY294002处理2 h后、再加入5μmol/L PNU共同处理18 h,免疫印迹法观察各组星形胶质细胞内HSF70蛋白的表达。结果:与正常对照组相比,PNU处理组星形胶质细胞内HSP70蛋白的表达上调(P <0. 05);与PNU处理组比较,LY294002+PNU处理组星形胶质细胞内HSP70表达明显受到抑制(P <0. 01)。结论:PNU通过PI3K/AKT信号通路调控星形胶质细胞HSP70蛋白表达。Objective:To study the effect of PNU on the expression of heat shock transcription factor 1(HSF1)in astrocytes and its action mode.Methods:Primary rat astrocytes were isolated from newborn rats within 24 h,cultured and verified as astrocytes.After astrocytes became mature,they were divided into four groups:control group,PNU group,LY294002(PI3K/AKT inhibitor)group and the PNU+LY294002 group.The control group was untreated;the PNU group was given 5μmol/L PNU for 6 h,12 h,18 h and 24 h,respectively;LY294002 group was treated with 10μmol/LLY294002;PNU+LY294002 group was pretreated with 10 umol/L LY294002 for 2 h and then given 5μmol/L PNU for 18 h.HSP70 expression levels were detected by Westerm Blot.Results:HSP70 level in PNU group was upregulated(P<0.05)when compared with the control group.LY294002 significantly inhibited PNU-upregulated HSP70 expression(P<0.01).Conclusion:PI3K/AKT pathway mediates PNU-upregulates HSP70 expression in astrocytes.

关 键 词：原代星形胶质细胞 热休克蛋白70 PNU PI3 K/AKT信号通路 阿尔茨海默症 

分 类 号：R749.16[医药卫生—神经病学与精神病学]