冬虫夏草菌分批发酵动力学模型的研究  

Kinetic Model of Batch Fermentation of Cordyceps sinensis

作　　者：方苏[1,2] 周立彪 闵少坤[1,2] 徐慧中 FANG Su;ZHOU Li-biao;MIN Shao-kun;XU Hui-zhong(College of Biological Science and Engineering,Beifang University of Nationalities,Yinchuan 750021,China;Key Laboratory of Fermentation, Brewing Engineering and Biotechnology, National Ethnic Affairs Commission,Yinchuan 750021,China)

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021 [2]国家民委发酵酿造工程生物技术重点实验室,宁夏银川750021

出　　处：《畜牧与饲料科学》2019年第3期1-5,共5页Animal Husbandry and Feed Science

基　　金：宁夏回族自治区科技惠民项目(2015KJHM41)

摘　　要：[目的]建立冬虫夏草菌分批发酵动力学模型。[方法]利用7 L机械搅拌式发酵罐对冬虫夏草菌进行分批培养,对菌体、胞外产物和底物的代谢过程进行分析。基于Logistic方程以及Luedeking-Piret方程等,通过1stOpt软件对试验数据进行非线性拟合及相关参数求解,建立菌体生长、胞外虫草酸(D-甘露醇)合成及糖消耗的发酵动力学模型。[结果]经拟合得到的模型参数估算值基本符合发酵规律,3项模型联立拟合R^2为0.986 5,菌体、产物和底物拟合曲线R^2分别为0.971 9、0.988 0和0.991 7。[结论]模型拟合良好,能够反映冬虫夏草菌发酵动力学特性。[Objective] To establish a kinetic model of batch fermentation of Cordyceps sinensis.[Method] Batch culture of Cordyceps sinensis were performed with a 7 L mechanically stirred fermenter, and the metabolic processes of the cells, extracellular products and substrates were analyzed.Based on Logistic equation and Luedeking-Piret equation, the non-linear fitting and related parameters solving were carried out by using 1stOpt software to establish a fermentation kinetic model of cell growth, extracellular oxalic acid (D-mannitol) synthesis and sugar consumption.[Result] The estimated model parameters obtained by fitting were basically consistent with the fermentation law.The simultaneous fitting R2 of the three separate models was 0.986 5, and R2 of the fitting curves of the cells, products and substrates are 0.971 9, 0.988 0 and 0.991 7, respectively.[Conclusion] The model fits well and can characterize the fermentation kinetic of Cordyceps sinensis.

关 键 词：冬虫夏草菌 分批发酵 动力学模型 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]