温度诱导对鼠李糖乳杆菌cspc mRNA基因表达和生长速率的影响  

Effect of Temperature Treatment on the Expression of cspc mRNA and Growth Rate of Lactobacillus rhamnosus

作　　者：董至恒 郭楠 石瑞文 包小妹 王玉华 张烨 关海滨 DONG Zhi-heng;GUO Nan;SHI Rui-wen;BAO Xiao-mei;WANG Yu-hua;ZHANG Ye;GUAN Hai-bin(College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China;Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010059,China)

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010110 [2]内蒙古医科大学附属医院药剂部,内蒙古呼和浩特010059

出　　处：《食品工业科技》2019年第6期137-140,共4页Science and Technology of Food Industry

基　　金：内蒙古自治区高等学校科学技术研究项目(NJZY14139);内蒙古医科大学2018年度大学生科技创新"英才培育"立项项目(YCPY2018048);内蒙古医科大学2018年度大学生创新创业训练计划校级项目(2018101320034)

摘　　要：通过在发酵过程中对鼠李糖乳杆菌(LGG)进行温度诱导处理,考察其冷休克蛋白C(cold shock proteins C,cspc) mRNA基因的表达和菌体生长速率的变化。在不同温度、不同时间条件下进行诱导,检测cspc mRNA基因的表达量;在发酵培养的第4、8 h温度诱导菌体,检测其生长速率,并且考察其诱导后菌体的冻干存活率。结果表明:高于37℃温度诱导可使cspc mRNA基因的表达量明显降低,且经43℃、30 min诱导后cspc mRNA基因的表达量最低,并且在生长过程中进行43℃、30 min诱导后菌体的生长速率明显提高且菌体的冻干存活率未降低。综上,高于37℃温度诱导可降低LGG的cspc mRNA基因表达量并提高生长速率。Through the method of temperature treatment on Lactobacillus rhamnosus (LGG) fermentation process,the expression of cold shock protein C (cspc) mRNA had been investigated,and the change of growth rate was investigated. With different temperature and time conditions,cspc mRNA expression was detected. The LGG was induced at the 4th and 8th hours with temperature in fermentation culture,growth rateswere detected,and the freeze-drying survival rate was tested. The results showed that the cspc mRNA expression was significantly decreased by temperature higher than 37 ℃ induction,and the expression was lowest after induction at 43 ℃ for 30 min,the growth rate was significantly increased after induction,the freeze-dried survival rate was not decreased.In summary,temperature higher than 37 ℃ induction could decrease the expression of cspc mRNA and increase the growth rate of LGG.

关 键 词：鼠李糖乳杆菌 冷休克蛋白C 温度诱导 生长速率 

分 类 号：TS201.3[轻工技术与工程—食品科学]