流动注射化学发光法在线监测饮用水中赭曲霉毒素A突发污染  

On-line monitoring of emergency pollution of ochratoxin A in drinking water by flow injection chemiluminescence

作　　者：李梁 唐书泽[1] 杨盼盼 刘倩妤 吴事正 LI Liang;TANG Shu-ze;YANG Pan-pan;LIU Qian-yu;WU Shi-zheng(Department of Food Science and Engineering,Jinan University,Guangzhou,Guangdong510632,China)

机构地区：[1]暨南大学食品科学与工程系,广东广州510632

出　　处：《食品与机械》2019年第1期86-91,104,共7页Food and Machinery

基　　金：粤港联合创新项目(编号:2016A050503031)

摘　　要：基于赭曲霉毒素A对鲁米诺—高碘酸钠—吐温20发光体系的抑制作用,建立了快速在线监测赭曲霉毒素A的新方法。经响应面优化试验条件后,该方法的线性范围为0.006～1.000μg/mL;检出限为1.74×10^(-3)μg/mL(S/N=3);加标回收率为80.6%～92.4%。对浓度0.02μg/mL赭曲霉毒素A平行测定11次,相对标准偏差(RSD)为1.68%。该方法快捷简便,可用于饮用水中赭曲霉毒素A突发污染的快速在线检测和预警。Based on quenching effect of ochratoxin A on chemiluminescence(CL)reaction of luminol-NaIO4 system catalyzing by Tween 20,a novel flow-injection chemiluminescence method was developed for the rapid on-line determination of possible sudden pollution of ochratoxin A in drinking water.Under the optimum conditions of response surface experiment,the linear range for ochratoxin A was 0.006~1.000μg/mL with a detection limit of 1.74×10^-3μg/mL and recovery of 80.6%~92.4%.The relative standard deviation(RSD)was 1.68% by analyzing samples containing 0.02μg/mL ochratoxin A(n=11).This method was founs convenient to operate,and couls be applied to the rapid emergency warning and real-time monitoring of ochratoxin A in drinking water.

关 键 词：赭曲霉毒素A 化学发光 流动注射 在线监测 

分 类 号：TU991.2[建筑科学—市政工程] O657[理学—分析化学]