李品种资源果实抗氧化活性分析  

Antioxidant capacity in fruits of cultivar resources of genus Prunus

作　　者：马小雪[1] 章秋平[1] 刘威生[1] 刘宁[1] 张玉萍[1] 徐铭[1] 刘硕[1] 张玉君[1] MA Xiaoxue;ZHANG Qiuping;LIU Weisheng;LIU Ning;ZHANG Yuping;XU Ming;LIU Shuo;ZHANG Yujun(Liaoning Institute of Pomology,Yingkou 115009,Liaoning,China)

机构地区：[1]辽宁省果树科学研究所,辽宁营口115009

出　　处：《果树学报》2019年第3期277-285,共9页Journal of Fruit Science

基　　金：农业部物种品种资源保护项目(111821301354052003);国家科技资源共享服务平台项目(NICGR2018-56);辽宁省北方果树资源与育种重点实验室(2018103002)

摘　　要：【目的】为李种质资源功能物质的科学评价与高抗氧化活性种质筛选提供基础数据。【方法】对李7个主要种和5份李杏杂交种等59份代表种质的清除DPPH自由基能力、清除ABTS自由基能力、还原Fe^(3+)能力(FRAP)和总酚含量进行检测。【结果】供试品种的抗氧化活性和总酚含量变异丰富,果实抗氧化活性与总酚含量呈显著相关,且不同种间或类型间存在显著差异。其中,美洲李(P. americana)的抗氧化活性和总酚含量显著高于其他种,栽培欧洲李(P.domesitica)的抗氧化活性和总酚含量最低。不同颜色李品种抗氧化活性和多酚含量差异不显著。抗氧化活性和总酚含量均值最大的是红色和紫红色品种,最低的是绿色品种。抗氧化活性和总酚含量较高的中国李品种有‘美丽李‘’奎丽‘’蜜李‘’紫玉’和‘绥李3号’。【结论】李种质间抗氧化活性和总酚含量变异非常丰富;不同种或果实着色的李种质抗氧化活性存在一定的差异。【Objective】Antioxidant capacity of fruit has been confirmed to improve health and prevent many kinds of cancers and aging-related illness of people. Breeding fruit varieties with high antioxidant capacity is one of the most important aims of breeders. Plums are so riched in chemicals of high antioxidant capacity such as polyphenols and flavonoids that are more and more popular. Evaluaton of the antioxidant level of plum resources is fundamental work for breeding plum varieties with high polyphenols and antioxidant capacity. The aim of our study is to provide basic data for further evaluation of antioxidant capacity of plum resources, and to select plum germplasm resources with high antioxidant capacity and phenolic contents.【Methods】The total antioxidant capacity(TAC) and total phenolic content(TPC)of 59 accessions of plum including 7 Prunus species, that is, P. salicina, P. simonii, P. domesitica, P.americana, P. nigra, P. cerasifera, and P. spinosa, four wild types of P. domesitica and five P. salicina hybrids with different colors were analyzed. All the plum materials were sampled in Chinese Germplasm Repository for Plums and Apricots(Xiongyue town, Liaoning province) in 2015 during ripening period. The flesh(with skin) was separated from kernel, and then was cut up and was mixed. The samples were stored under-80 ℃ in ultra-low temperature freezer. TAC was analyzed using the following 3 methods: 2, 2-diphenyl-1-picrylhydrazyl radical scavenging capacity(DPPH), trolox equivalent antioxidant capacity(ABTS) and ferric ion reducing antioxidant power(FRAP). TPC was detected by Folin-Ciocalteau method.【Results】The plum resources had a wide range of TAC and TPC. The range of TAC(DPPH) was 4.58-17.36 μmol·g-1, and the most accessions of the cultivars were in the range of 9-10μmol·g-1. The range of TAC(ABTS) was 4.04-24.17 μmol·g-1, while TAC(FRAP) range was 9.74-75.61μmol·g-1. The TAC(FRAP) value was most in 10-35 μmol·g-1(43 accessions). The range of TPC was60.42-469.89 mg· 100 g-1, and the m

关 键 词：李 品种资源 抗氧化活性 总酚 果实着色 

分 类 号：S662.2[农业科学—果树学]