凝胶色谱法测定负载化硅胶的相对分子质量  

Determination of relative molecular weight of the loading silicone by gel permeation chromatography method

作　　者：刘颖慧 钮正睿[1] 曹进[1] LIU Ying-Hui;NIU Zheng-Rui;CAO Jin(National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《食品安全质量检测学报》2019年第1期123-128,共6页Journal of Food Safety and Quality

摘　　要：目的建立测定负载化硅胶的相对分子质量的凝胶色谱法。方法使用凝胶色谱法测定负载化硅胶的相对分子质量,选取已知不同分子量的聚乙二醇高聚物作为标准品,对检测结果进行标准曲线拟合,得到最佳拟合标曲,采用Shoedex SB-802 HQ凝胶色谱柱分离,示差折光检测器检测,测得准确负载化硅胶的相对分子质量。结果使用本方法测得未知负载化硅胶样品相对分子量为6.326×103,相对标准偏差(relative standard deviation, RSD)为1.45%。结论该方法简单、可靠,可用于测定负载化硅胶的相对分子质量。Objective To establish a method for the determination of relative molecular weight of the loading silicone by gel permeation chromatography(GPC) method.Methods The relative molecular weight of the loading silicone was determined by gel permeation chromatography.Polyethylene glycol(PEG) polymers with different molecular weights were selected as the standard material,and the test results were fitted with standard curve to obtain the optimal fitting standard curve.Shoedex sb-802 HQ gel chromatographic column was used for separation and the molecular weight of the loading silicone accurately was determined by differential refraction detector.Results By using this method,the relative molecular weight of the unknown loading silicone samples were 6.326×10^3,and the relative standard deviation(RSD) was 1.45%.Conclusion This method is simple and reliable,which can be used to determine the molecular weight of loading silicone.

关 键 词：负载化硅胶 聚乙二醇 示差折光检测器 

分 类 号：TS206.4[轻工技术与工程—食品科学] O657.7[轻工技术与工程—食品科学与工程]