MicroRNA-29a在结直肠癌组织中的表达及意义  

Expression and significance of miRNA-29a in colorectal cancer

作　　者：蔡锐文 梁伟成[1] 黄冀华[1] 梁华艳[1] 黄开劲 邓波[2] Rui-wen Cai;Wei-cheng Liang;Ji-hua Huang;Hua-yan Liang;Kai-jin Huang;Bo Deng(Department of Gastrointestinal Surgery,People's Hospital of Gaozhou,Gaozhou,Guangdong 525200,China;Department of Laboratory Medicine,People’s Hospital of Gaozhou,Gaozhou,Guangdong 525200,China)

机构地区：[1]广东省高州市人民医院胃肠外科,广东高州525200 [2]广东省高州市人民医院检验科,广东高州525200

出　　处：《中国现代医学杂志》2019年第7期43-47,共5页China Journal of Modern Medicine

摘　　要：目的探讨micro RNA-29a(mi R-29a)在结直肠癌组织中的表达,以及mi R-29a反义寡核苷酸对结直肠癌细胞增殖和侵袭的影响。方法选取行手术治疗的初诊结直肠癌患者195例,利用实时荧光定量聚合酶链反应检测结直肠癌和癌旁组织中mi R-29a表达,培养人结直肠癌Lo Vo细胞,随机分为ASO-mi R-29a组、ASO-对照序列组和空白对照组,检测各组细胞中mi R-29a表达,MTT法检测各组细胞增殖能力,Transwell小室检测各组细胞迁移和侵袭能力。结果结直肠癌组织mi R-29a相对表达量为(1.93±0.19),癌旁组织为(1.26±0.12),两者比较差异有统计学意义(P <0.05);结直肠癌组织中mi R-29a相对表达量与TNM分期和淋巴结转移有关(P <0.05),多元线性回归分析结果显示,TNM分期和淋巴结转移是影响结直肠癌组织中mi R-29a表达的因素(P <0.05);ASO-mi R-29a组细胞中mi R-29a相对表达量低于ASO-对照序列组和空白对照组(P <0.05);MTT实验结果显示,ASO-miR-29a组与ASO-对照序列组和空白对照组相比吸光度值降低(P <0.05);与ASO-对照序列组和空白对照组比较,ASO-miR-29a组迁移细胞数和侵袭细胞数均减少(P <0.05)。结论 mi R-29a在结直肠癌组织中呈高表达,参与结直肠癌发生、进展过程,特异性抑制结直肠癌细胞中miR-29a表达可减少细胞增殖,抑制细胞迁移和侵袭能力。Objective To investigate the expression of miRNA-29a in colorectal cancer,and the effect of miRNA-29a on proliferation and invasion of colorectal cancer cells.Methods A total of 195 cases of colorectal cancer were involved in this study.The expression of miRNA-29a in colorectal cancer tissue and adjacent normal tissue was detected by real-time fluorescence quantitative PCR.Human colorectal cancer LoVo cell line was cultured.All cells were randomly divided into ASO-miRNA-29a group,ASO-control sequence group and blank control group.MTT and Transwell assay were performed to detect the cell proliferation and invasion,respectively.Results The relative expression level of miRNA-29a in the colorectal cancer tissues was increased when compared with adjacent tissues was[(1.93±0.19)vs(1.26±0.12),P<0.05].Multiple linear regression analysis suggested that increased expression level of miRNA-29a in colorectal cancer tissues was correlated to TNM stage and lymph node metastasis(P<0.05).The relative expression level of miRNA-29a in ASO-miRNA-29a group was lower than the ASO-control group and blank control group(P<0.05).MTT and Transwell assay showed that silencing miRNA-29a compromised cellular proliferation and invasive capability when compared with the ASO-control sequence group and blank control group(P<0.05).Conclusions Enhanced expression of miRNA-29a in colorectal cancer tissues may be involved in the carcinogenesis and progression of colorectal cancer.

关 键 词：结直肠肿瘤 MICRORNAS 反义寡核苷酸 细胞增殖 细胞侵袭 

分 类 号：R735.3[医药卫生—肿瘤]