老年宫颈鳞癌患者HPV16/18感染与癌组织PDGFR-α和PDGFR-β蛋白表达的关系  

Relationship between HPV16/18 Infection and Expression of PDGFR-and PDGFR-beta Protein in Cancer Tissues of Elderly Patients with Cervical Squamous Cell Carcinoma

作　　者：张玉芳 任春丽[1] 侯敬[1] 张金环[1] 郭艳巍[1] 唐明霞 ZHANG Yufang;REN Chunli;HOU Jing(The Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China)

机构地区：[1]承德医学院附属医院妇产科,河北承德067000 [2]河北省承德市人民政府机关门诊部,河北承德067000

出　　处：《河北医学》2019年第4期543-547,共5页Hebei Medicine

基　　金：河北省承德市科技支撑计划项目;(编号:201606A047)

摘　　要：目的:在老年宫颈鳞癌患者中探索HPV16/18感染与癌组织血小板衍生生长因子受体α(platelet-derived growth factor receptor-alpha,PDGFR-α)和血小板衍生生长因子受体β(platelet-derived growth factor receptor-alpha,PDGFR-β)蛋白表达的关系以及三者之间的相互关系。方法:选取老年宫颈鳞癌患者75例、正常子宫颈组织32例,分别采用原位杂交技术检测HPV16/18感染,用免疫组织化学染色检测PDGFR-α和PDGFR-β蛋白。结果:老年宫颈鳞癌患者中HPV16/18感染率高达69.33%(52/75),而正常对照组为18.75%(6/32),老年宫颈鳞癌患者HPV16/18的感染率均显著高于对照组(P<0.05)。在对照组中检测到1例PDGFR-α蛋白的表达,表达阳性率3.13%,而老年宫颈鳞癌患者组织中检测到59例PDGFR-α蛋白的阳性表达,表达阳性率78.67%,差异有统计学意义(P <0.05);PDGFR-α蛋白的阳性表达率与宫颈鳞癌患者临床分期有关(P <0.05);在对照组中PDGFR-β蛋白阳性表达率较低为6.25%,而宫颈鳞癌患者组织中PDGFR-β蛋白表达率较高为62.67%,差异有统计学意义(P<0.05); PDGFR-β蛋白表达量与宫颈鳞临床分期(P<0.05);在72例宫颈鳞癌组织中,HPV16/18感染阳性患者共有47例,表达阳性率62.67%,其中PDGFR-α蛋白阳性有45例,表达阳性率95.74%,PDGFR-β蛋白阳性有40例,表达阳性率85.11%,显著高于未感染HPV16/18的宫颈鳞癌患者,差异有统计学意义(P<0.05),Spearman相关性分析发现PDGFR-α与PDGFR-β蛋白的表达与宫颈癌患者HPV16/18感染呈显著正相关(rs=0.428,P <0.05; rs=0.562,P <0.05);此外,在宫颈癌患者中,PDGFR-α与PDGFR-β蛋白表达水平也呈显著正相关(rs=0.639,P <0.05)。结论:老年宫颈鳞癌的发生与HPV16/18感染关系密切,在HPV16/18感染的宫颈鳞癌组织中PDGFR-α与PDGFR-β蛋白表达水平显著增高,并且在宫颈鳞癌发生、发展中起着协同作用,共同促进宫颈鳞癌的发展。Objective: The relationship between the expression of HPV16/18 infection and the expression of platelet derived growth factor receptor alpha (platelet-derived growth factor receptor-alpha, PDGFR- a) and platelet derived growth factor receptor beta (platelet-derived growth factor receptor-alpha, PDGFR- beta) in the elderly patients with squamous cell carcinoma of the cervix and the expression of platelet derived growth factor receptor beta (platelet-derived growth factor receptor-alpha, PDGFR- beta) and the relationship between the three The relationship between each other. Methods: 75 cases of senile cervical squamous cell carcinoma and 32 of normal cervical tissue were selected. In situ hybridization was used to detect HPV16 /18 infection, and PDGFR- A and PDGFR- beta protein were detected by immunohistochemistry. Results: The infection rates of HPV16 /18 in cervical squamous cell carcinoma group and control group were 69.33%(52 /75) and 18.75%(6 /32) respectively.The infection rate of HPV16 /18 in elderly patients with cervical squamous cell carcinoma was significantly higher than that in the control group (P <0. 05). One case of PDGFR-α protein expression was detected in the control group, Positive expression rate 3.13%. In the elderly patients with cervical squamous cell carcinoma, 59 cases of PDGFR-α protein were detected positively, Positive expression rat 78.67%, and the difference was statistically significant.(P<0.05);The positive expression rate of PDGFR-α protein was related to the clinical stage of cervical squamous cell carcinoma patients (P<0.05);The positive expression rate of PDGFR-β protein was lower in the control group was 6.25%, and the cervical squamous cell carcinoma patients The expression rate of PDGFR-β protein in tissues was 62.67%, and the difference was statistically significant (P<0.05). The expression of PDGFR-β protein was correlated with the clinical stage of cervical scales (P<0.05);Among 72 cases of cervical squamous cell carcinoma, 47 cases (Positive expression rat 62.67%)

关 键 词：老年宫颈鳞癌 HPV16/18 PDGFR-Α PDGFR-Β 

分 类 号：R737.33[医药卫生—肿瘤]