No detrimental effect of Bt maize pollen containing Cry1Ab/2Aj or Cry1Ac on adult green lacewings Chrysoperla sinica Tjeder  

作　　者：LIU Yan-min LI Yun-he CHEN Xiu-ping SONG Xin-yuan SHEN Ping PENG Yu-fa 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences [2]Jilin Academy of Agricultural Sciences [3]Science and Technology Development Center, Ministry of Agriculture

出　　处：《Journal of Integrative Agriculture》2019年第4期893-899,共7页农业科学学报（英文版）

基　　金：supported by the National GMO New Variety Breeding Program of China(2015ZX08013-003)

摘　　要：Adult Chrysoperla sinica Tjeder is a common pollen feeder in maize fields. They are thus directly exposed to insecticidal proteins by consumption of genetically engineered maize pollen containing Bacillus thuringiensis(Bt) proteins. Here we assessed the potential effects of Cry1 Ab/2 Aj-or Cry1 Ac-containing Bt maize pollen on the fitness of adult C. sinica via a dietary-exposure assay under laboratory conditions. Survival, pre-oviposition, fecundity and adult dry weight did not differ between adult C. sinica consuming Bt or the corresponding non-Bt maize pollen. The stability of the Cry protein in the food sources and uptake of the Cry protein by adult C. sinica during the feeding experiment were confirmed by ELISA. These results demonstrate that adult C. sinica are not affected by the consumption of Cry1 Ab/2 Aj-or Cry1 Ac-containing maize pollen, suggesting that production of Bt maize expressing cry1 Ab/2 Aj or cry1 Ac genes will pose a negligible risk to adult C. sinica.Adult Chrysoperla sinica Tjeder is a common pollen feeder in maize fields. They are thus directly exposed to insecticidal proteins by consumption of genetically engineered maize pollen containing Bacillus thuringiensis(Bt) proteins. Here we assessed the potential effects of Cry1 Ab/2 Aj-or Cry1 Ac-containing Bt maize pollen on the fitness of adult C. sinica via a dietary-exposure assay under laboratory conditions. Survival, pre-oviposition, fecundity and adult dry weight did not differ between adult C. sinica consuming Bt or the corresponding non-Bt maize pollen. The stability of the Cry protein in the food sources and uptake of the Cry protein by adult C. sinica during the feeding experiment were confirmed by ELISA. These results demonstrate that adult C. sinica are not affected by the consumption of Cry1 Ab/2 Aj-or Cry1 Ac-containing maize pollen, suggesting that production of Bt maize expressing cry1 Ab/2 Aj or cry1 Ac genes will pose a negligible risk to adult C. sinica.

关 键 词：non-target effect environmental risk assessment ELISA DIETARY exposure ASSAY 

分 类 号：S[农业科学]