Fmoc-苯丙氨酸水凝胶及其复配EGCG抑菌作用研究  

Fmoc-Phenylalanine Hydrogel and Its Complexed with EGCG Used for Antibacterial Activity

作　　者：付成 袁发浒[2] 胡芸瑕 高连君 魏栋玉 陈春兰 FU Cheng;YUAN Fa-hu;HU Yun-xia;GAO Lian-jun;WEI Dong-yu;CHEN Chun-lan(Institute for Interdisciplinary Research,Jianghan University,Wuhan 430056,Hubei,China;School of Medicine,Jianghan University,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学交叉学科研究院,湖北武汉430056 [2]江汉大学医学院,湖北武汉430056

出　　处：《食品研究与开发》2019年第10期37-42,共6页Food Research and Development

基　　金：湖北省卫生和计划生育委员会科研项目(WJ2017X016)

摘　　要：以pH 7.4的磷酸盐缓冲液体系制备9-芴甲氧羰基-苯丙氨酸(N-[(9H-fluoren-9-ylmethoxy)carbonyl]-3-phenylalanine,Fmoc-Phe)水凝胶,对比研究D型Fmoc-苯丙氨酸(Fmoc-DPhe)和L型Fmoc-苯丙氨酸(Fmoc-LPhe)形成的水凝胶特性及抑菌性能,并分析Fmoc-DPhe与表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)复配的协同抑菌性。结果表明,D型和L型的Fmoc-Phe在质量浓度高于0.2%、0.2 mol/L磷酸盐缓冲液时可以形成透明的水凝胶,且二者均对金黄色葡萄球菌有明显的抑制效果;随着Fmoc-Phe的浓度增加,抑菌效果随之增强。将12 mg/mL的Fmoc-DPhe与5mg/mL的EGCG复配,发现二者复配形成的水凝胶能够起到协同抑制金黄色葡萄球菌生长的结果。12 mg/mL Fmoc-Phe形成的水凝胶,其性状在10个月的研究期限内可维持稳定。初步探讨水凝胶的抑菌机理,发现与Fmoc-Phe具有类似结构的Fmoc-酪氨酸(Fmoc-LTyr)也具有抑制金黄色葡萄球菌生长的效果,表明Fmoc修饰的氨基酸的抑菌能力是由苯环母体经Fmoc基团修饰后产生的。Fmoc-phenylalanine (Fmoc-Phe) supramolecular hydrogel was prepared with the phosphate buffer at pH 7.4. The hydrogel characteristics and antibacterial properties of D-type Fmoc-phenylalanine (Fmoc-DPhe) and L-type Fmoc-phenylalanine (Fmoc-LPhe) were compared, and the synergistic antibacterial properties of Fmoc-DPhe and epigallocatechin gallate (EGCG) were analyzed. The results showed that the D-type and L-type Fmoc-LPhe could form transparent hydrogel when the mass concentration was higher than 0.2 % and the concentration of phosphate buffer at 0.2 mol/L, and both of them had obvious inhibition effect on Staphylococcus aureus. With the increase of Fmoc-Phe concentration, the antibacterial effect was enhanced. When 12 mg/mL of Fmoc-DPhe was combined with 5 mg/mL of EGCG, it was found that the hydrogel formed by the combination of the two compounds could synergistically inhibit the growth of Staphylococcus aureus. Hydrogel formed by 12 mg/mL Fmoc-Phe were stable during the 10-month study period. It was found that Fmoc-LTyr, which had a similar molecular structure to Fmoc-Phe, also had the effect of inhibiting the growth of Staphylococcus aureus, indicating that the bacteriostatic capacity of Fmoc-modified amino acids was generated by the phenyl group modified by Fmoc group.

关 键 词：超分子水凝胶 对映异构体 抑菌性 表没食子儿茶素没食子酸酯(EGCG) 复配 

分 类 号：TQ427.26[化学工程] TS201.3[轻工技术与工程—食品科学]