高产纤维素酶真菌的筛选及鉴定  

Screening and identification of high-level cellulase-producing fungi

作　　者：李豪[1] 邹伟[1,2] 白光剑 徐静 LI Hao;ZOU Wei;BAI Guangjian;XU Jing(Sichuan University of Science & Engineering, College of Bioengineering, Zigong 643000,China;Sichuan University of Science & Engineering, Liquor-Makign Bio-Technology &Application of Key Laboratory of Sichuan Province, Zigong 643000,China)

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]酿酒生物技术及应用四川省重点实验室(四川理工学院),四川自贡643000

出　　处：《食品与发酵工业》2019年第6期54-58,共5页Food and Fermentation Industries

基　　金：四川理工学院研究生创新基金项目(y2017043);四川省大学生创新创业训练计划项目(201710622036);四川省教育厅项目(15ZB0204);酿酒生物技术及应用四川省重点实验室项目(NJ2014-08);自贡市科技局创新苗子工程项目(2018CXMZ02)

摘　　要：该研究旨在从腐殖土中筛选得到高产纤维素酶的菌株。利用刚果红染色作为初筛,菌株酶活测定作为复筛得到3株高产纤维素酶的菌株。通过形态学鉴定与分子生物学鉴定,菌株B03、E15为枝孢菌属(Cladosporium sp.),D11为极细枝孢菌(Cladosporium tenuissimum)。菌株B03在以CMC为底物,28℃、180 r/min条件下培养3 d后,CMC酶活达(427. 62±2. 78) U/m L,FPA酶活为(46. 83±1. 85) U/m L,在以油菜秸秆为底物时其CMC酶活也达到(352. 50±4. 17) U/m L,培养6 d后秸秆降解率达26. 8%,具有较好的降解能力。筛选得到3株高产纤维素酶的真菌菌株,其中菌株B03的纤维素酶活较高,是一株具有研究潜力的纤维素酶菌株。This study aimed to screen high-level cellulase-producing fungi from humus soil. By primary screening on plates with Congo red staining followed by enzyme activity assay,three isolates were obtained and identified as Cladosporium sp.( isolates B03 and E15) and Cladosporium tenuissimum( D11) based on their morphology and molecular markers. The strain B03 produced cellulase with(427. 62 ± 2. 78) U/m L against CMC and(46. 83 ± 1. 85) U/m L against FPA after being cultured for 3 d at 28℃ and at 180 r/min using CMC as the substrate. By using rape straw as the substrate,it produced(352. 50 ± 4. 17) U/m L CMC activity and degraded 26. 8% of the straw after culturing for6 d. In conclusion,strain B03 produced relatively high-level of cellulose among three selected isolates,which is worth for further study.

关 键 词：纤维素酶 筛选 枝孢菌 

分 类 号：Q93[生物学—微生物学]