Regulation of osteoblast functions on titanium surfaces with different micro/nanotopographies and compositions  

作　　者：HE Peng WANG XiaoLan NING ChengYun LIU XiaoWei LI Mei XU HaiDong GUO GuoDong MAO GuangPing LIU Gang XU Bin ZHANG Yu ZHAO JianNing 

机构地区：[1]Department of Orthopedics, Nanjing General Hospital of Nanjing Military Command of PLA [2]Department of Orthopedics, Guangdong General Hospital, Guangdong Academy of Medical Sciences [3]School of Materials Science and Engineering, South China University of Technology

出　　处：《Science China(Technological Sciences)》2019年第4期559-568,共10页中国科学（技术科学英文版）

基　　金：supported of by the National Key Research and Development Program of China(Grant No.2016YFB0700803);the National Natural Science Foundation of China(Grant Nos.81501925,81501859,31700880);the Natural Science Foundation of Guangdong Province(Grant No.2015A030312004);the Science and Technology Planning Project of Guangzhou city(Grant No.201604020110)

摘　　要：Surface modification of medical implants is considered as an effective method to improve cellular behaviors and the integration of tissues with materials. Titanium(Ti)-based materials with four different micro/nano-structures and compositions were prepared by acid etching, electrochemical anodization and alkali-heat treatment. The surface morphologies and compositions of the different surface-modified Ti materials were characterized by field-emission scanning electron microscopy(FE-SEM),atomic force microscopy(AFM) and X-ray diffraction(XRD). The effects of the micro/nano structured and compositions of the surfaces on cellular responses were investigated in vitro by observing the morphology, adhesion, proliferation and osteogenic differentiation of osteoblasts. To further investigate the underlying mechanisms, an RT-PCR assay was performed to analyze the expression levels of cell adhesion-related genes. Our results indicated that the nanosized structure and anatase composition could promote the adhesion and proliferation of MC3T3-E1 pre-osteoblast, as well as alkaline phosphatase activity and extracellular matrix mineralization via the integrin-FAK signaling pathway. Taken together, our innovation presented in this work demonstrated that the surface nano-structure design and composition of biomedical implants can be modified of for future orthopaedic applications.Surface modification of medical implants is considered as an effective method to improve cellular behaviors and the integration of tissues with materials. Titanium(Ti)-based materials with four different micro/nano-structures and compositions were prepared by acid etching, electrochemical anodization and alkali-heat treatment. The surface morphologies and compositions of the different surface-modified Ti materials were characterized by field-emission scanning electron microscopy(FE-SEM),atomic force microscopy(AFM) and X-ray diffraction(XRD). The effects of the micro/nano structured and compositions of the surfaces on cellular responses were investigated in vitro by observing the morphology, adhesion, proliferation and osteogenic differentiation of osteoblasts. To further investigate the underlying mechanisms, an RT-PCR assay was performed to analyze the expression levels of cell adhesion-related genes. Our results indicated that the nanosized structure and anatase composition could promote the adhesion and proliferation of MC3T3-E1 pre-osteoblast, as well as alkaline phosphatase activity and extracellular matrix mineralization via the integrin-FAK signaling pathway. Taken together, our innovation presented in this work demonstrated that the surface nano-structure design and composition of biomedical implants can be modified of for future orthopaedic applications.

关 键 词：OSTEOBLAST FUNCTIONS micro/nanotopography composition TITANIUM 

分 类 号：TH[机械工程]