仿刺参胶原蛋白多肽对Ⅱ型糖尿病小鼠肾脏的保护机制  

Regulation of Apostichopus joponicus Polypeptide on Kidney of Type Ⅱ Diabetic Mice

作　　者：韩姣姣[1,2] 李妍妍 周君[1] 芦晨阳[1] 李晔[1] 张凌芷 苏秀榕[1] Han Jiaojiao;Li Yanyan;Zhou Jun;Lu Chenyang;Li Ye;Choeng Lingzhi;Su Xiurong(School of Marine Sciences , Ningbo University, Ningbo 315211, Zhejiang;College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, Zhejiang;King Abdullah University of Science and Technology, Jeddah, Saudi Arabia)

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]宁波大学食品与药学学院,浙江宁波315211 [3]阿卜杜拉国王科技大学

出　　处：《中国食品学报》2019年第3期108-114,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家农业科技成果转化项目(2007C22030520);浙江省重大科技专项重大农业项目(2008C02009-Ⅱ-2)

摘　　要：目的:研究仿刺参胶原蛋白多肽对db/db糖尿病小鼠的保护机制。方法:通过仿刺参多肽分组喂养糖尿病小鼠,测定小鼠血清中TC、TG、LDL-C和HDL-C含量及胰岛素浓度。采用双向电泳研究小鼠的肾脏蛋白,通过差异蛋白筛选,GO功能注释,代谢通路等分析确定仿刺参多肽对糖尿病小鼠肾脏的保护机制。生化指标显示:仿刺参多肽可以降低血清中TC、TG、LDL-C的含量和胰岛素浓度,提高HDL-C水平。双向结果表明:相对于模型组,试验组共检测出上调蛋白81个,下调蛋白51个。GO结果显示:差异蛋白的功能主要集中在蛋白转运和信号传导过程。KEGG分析发现,差异蛋白主要富集在代谢途径、胰岛素抵抗和胰岛素信号传导过程。结论:喂养仿刺参多肽的小鼠体内视黄醇结合蛋白4的表达下调,血糖水平下降,胰岛素信号通路恢复正常,可以有效缓解糖尿病的症状。The purpose of this paper is to study the protection mechanism of Apostichopus joponicus polypeptide to Ⅱ diabetic mouse(db/db diabetic mouse) kidney. By animal experiments, db/db mice are fed in different groups. Then determinati the content of TC, TG, LDL-C, HDL-C and insulin concentration in mouse serum. The regulation of db/db diabetic mouse kidney is studied by two-dimensional electrophoresis. Then detecting differential proteins of db/db mice kidneys, differential protein expression, GO functional analysis and KEGG pathway analysis. Apostichopus joponicus polypeptide can reduce the levels of serum TC, TG, LDL and insulin concentration, increased HDL-C levels in diabetic mouse. Two-dimensional electrophoresis results showed that compared with the model group, the number of up-regulated protein is 81, and down-regulated protein is 51 in the experimental group. GO results showed that the function of the differential protein mainly focused on protein transport and signal transduction. KEGG analysis revealed that the differential proteins were mainly enriched in metabolic pathways, insulin resistance and insulin signaling processes. The expression of retinol-binding protein 4 in mice fed with polypeptides was reduced, the blood glucose level was decreased, and the insulin signal pathway returned to normal, which could relieve the symptoms of diabetes mellitus.

关 键 词：仿刺参多肽 Ⅱ型糖尿病 双向电泳 差异蛋白 

分 类 号：R965[医药卫生—药理学]