Argininosuccinate synthase 1 suppresses cancer cell invasion by inhibiting STAT3 pathway in hepatocellular carcinoma  

作　　者：Xuemei Tao Qiaozhu Zuo Haoyu Ruan Hui Wang Haojie Jin Zhuoan Cheng Yuanyuan Lv Wenxin Qin Cun Wang 

机构地区：[1]State Key Laboratory of Oncogenes and Related Genes,Department of Tumor Microenvironment,Shanghai Cancer Institute,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200032,China [2]Department of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Acta Biochimica et Biophysica Sinica》2019年第3期263-276,共14页生物化学与生物物理学报（英文版）

基　　金：the grants from the National Key Basic Research Program of China(No.2015CB553905);the National Natural Science Foundation of China(Nos.81201626,81402278,81421001,81572311,81672933,and 81772461);the National Key Sci-Tech Special Projects of Infectious Diseases(No.2018ZX10732202-002-003);the Project of Renji Hospital(No.PYXJS16-004);Shanghai Jiao Tong University School of Medicine(No.YG2014MS44);and Shanghai Municipal Commission of Health and Family Planning(No.201640007).

摘　　要：Metastasis is the main reason for high recurrence and poor survival of hepatocellular carcinoma(HCC).The molecular mechanism underlying HCC metastasis remains unclear.In this study,we found that argininosuccinate synthase 1(ASS1)expression was significantly decreased and down-regulation of ASS1 was closely correlated with poor prognosis in HCC patients.DNA methylation led to the down-regulation of ASS1 in HCC.Stable silencing of ASS1 promoted migration and invasion of HCC cells,whereas overexpression of ASS1-inhibited metastasis of HCC cells in vivo and in vitro.We also revealed that ASS1-knockdown increased the phosphorylation level of S727STAT3,which contributed to HCC metastasis by up-regulation of inhibitor of differentiation 1(ID1).These findings indicate that ASS1 inhibits HCC metastasis and may serve as a target for HCC diagnosis and treatment.

关 键 词：ASS1 HEPATOCELLULAR carcinoma prognosis METASTASIS 

分 类 号：Q[生物学]