青砖茶粗多糖抑制α-葡萄糖苷酶活性的研究  

Inhibitory effects of crude polysaccharide of green brick tea on α-glucosidase activity

作　　者：舒婷 肖畅 何慧 马蔚[1,2] 吕帮玉 杨新河[1,3,4] SHU Ting;XIAO Chang;HE Hui;MA Wei;LYU Bangyu;YANG Xinhe(School of Life Science and Technology, Hubei Engineering University, Xiaogan 432000;School of Life Science, Hubei University, Wuhan 430062;Institute of New Rural Development of Hubei Province, Xiaogan 432000;Hubei Province Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients,Xiaogan 432000)

机构地区：[1]湖北工程学院生命科学技术学院,孝感432000 [2]湖北大学生命科学学院,武汉430062 [3]湖北省新农村发展研究院,孝感432000 [4]湖北省植物功能成分利用工程技术研究中心,孝感432000

出　　处：《食品科技》2019年第3期194-199,共6页Food Science and Technology

基　　金：国家自然科学基金项目(31370692)

摘　　要：采用光谱和酶动力学方法研究青砖茶粗多糖对α-葡萄糖苷酶活性的抑制作用及机制。结果表明:青砖茶粗多糖对α-葡萄糖苷酶的抑制作用接近于阳性对照品阿卡波糖,二者的半抑制浓度依次为(2.04±0.07)mg/m L和(2.00±0.03)mg/m L,不存在显著性差异(P>0.05);Lineweaver-Burk双倒数作图显示青砖茶粗多糖的抑制类型为竞争性抑制,且紫外吸收光谱实验表明青砖茶粗多糖与α-葡萄糖苷酶之间存在相互作用。文章为深入开展青砖茶粗多糖降血糖活性研究及其功能性产品开发提供了理论基础。The inhibitory effects of crude polysaccharide of green brick tea on α-glucosidase were investigated by spectroscopy and enzyme kinetics, and the possible mechanisms involved were explored.The results showed that the inhibition activity of crude polysaccharide of green brick tea was close to that of acarbose as a positive control, and the half inhibitory concentration(IC50) values of crude polysaccharide and acarbose were(2.04±0.07)mg/mL and(2.00±0.03)mg/mL, respectively, and there is no significant difference between their IC50 values(P>0.05). The Lineweaver-Burk double reciprocal plot indicated that the inhibition type of crude polysaccharide of green brick tea was competitive inhibition,and Ultraviolet absorption spectra showed that there is interaction between crude polysaccharide of green brick tea and α-glucosidase. The results of the study provided the basis for further research on hpyerglycemic activity of crude polysaccharide of green brick tea as well as their functional product development.

关 键 词：青砖茶粗多糖 Α-葡萄糖苷酶 抑制作用 

分 类 号：R284.2[医药卫生—中药学]