菊糖作为结核分枝杆菌和人乳头瘤病毒疫苗佐剂的免疫原性研究  

Immunogenicity of inulin as vaccine adjuvant of human papillomavirus and tuberculosis

作　　者：胡瞬[1,2,3] 胡涛 贺丽萍[3] 易有金 HU Shun;HU Tao;HE Li-ping;YI You-jin(College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China)

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128 [2]中国科学院过程工程研究所,北京100190 [3]湘南学院公共卫生学院,湖南郴州423000

出　　处：《现代预防医学》2019年第8期1478-1482,共5页Modern Preventive Medicine

基　　金：国家自然科学基金(81703445)

摘　　要：目的研究菊糖的免疫佐剂效果,探讨菊糖与结核分枝杆菌(Mtb)ESAT-CFP10融合蛋白EC、人乳头瘤病毒16型E6-E7融合蛋白E6E7联合免疫小鼠的免疫应答特征。方法将菊糖(Inulin)分别与E6E7、EC共价结合制备E6E7-I、EC-I疫苗,采用E6E7-I、EC-I免疫小鼠,通过比较小鼠血清中抗原特异性IgG滴度,脾淋巴细胞抗原特异性IFN-γ、TNF-α分泌水平和CD4^+T、CD8^+T亚群分布,评价菊糖的疫苗佐剂功能。结果 (1)第7、14、21 d,菊糖结合的EC-I、E6E7-I组抗原特异性抗体IgG滴度分别高于单独抗原EC组、E6E7组(P<0.05),且未检出抗菊糖抗体。(2)EC-I、E6E7-I能分别诱导出高于EC组、E6E7的脾淋巴细胞IFN-γ、TNF-α分泌水平(P<0.05)。(3)EC-I组、EC组、PBS组的方差分析结果表明,EC-I组CD8^+T、CD4^+T细胞亚群所占百分比高于EC组(P<0.05;P=0.017);E6E7-I组的CD8^+T、CD4^+T细胞亚群所占百分比高于E6E7-I组,分别(P=0.007;0.016)。结论菊糖佐剂共价结合E6E7、EC蛋白,能有效激活机体的抗原特异性体液免疫和细胞免疫,且佐剂自身免疫原性低,提示菊糖作为HPV16型宫颈癌疫苗、TB疫苗佐剂具有良好的佐剂效应。Objective The aim of this study was to evaluate the immunogenicity and adjuvant effect of Jerusalem artichoke polysaccharide as vaccine adjuvant, and improve the immunogenicity of human papilloma carcinogenic antigen E6E7 fusion protein and tuberculous bacillus antigen EC fusion protein. Methods Inulin was combined with E6E7 and EC respectively to prepare I-E6E7 and I-EC as antigens and immunized mice, respectively. Then the immune response characteristics of mice were investigated. The specific IgG antibody titer, antigen specific IFN-γ, TNF-α expression in the mouse spleen lymphocyte were detected. The rations of CD4^+T cells and CD8^+T cells in spleen lymphocyte upon restimulation with EC or E6E7 in vitro and serum biochemical indexes were detected. Results The research showed that when inulin was used as an adjuvant to modify the antigen protein, at first, it could improve the antibody titer of the antigen protein, and fail to detect the anti-polysaccharide IgG antibody(P<0.05). And secondly, the higher secretion levels of IFN-γ and TNF-α than those of the control group were induced(P<0.05). At last, the proportion of CD4^+T and CD8^+T subgroups of spleen lymphocytes were significantly increased(P<0.05). Conclusion As a vaccine adjuvant, inulin covalent binding antigen activated the cellular and humoral immunity of the body, and its immunogenicity was weak. It may have good efficacy.

关 键 词：菊糖 佐剂 免疫原性 人乳头状瘤病毒 结核分枝杆菌 

分 类 号：R186[医药卫生—流行病学]