高产胞外多糖的空间诱变植物乳杆菌抗大鼠脂质过氧化功效研究  

Study on Lipid Peroxidation in Rats Induced by a Space-mutagenesis Lactobacillus Plantarum with High Yield of Extracellular Polysaccharide

作　　者：杨飞宇 张红星 谢远红 郝红炜 刘长庭[3] 刘慧 Yang Feiyu;Zhang Hongxing;Xie Yuanhong;HaoHongwei;Liu Changting;Liu Hui(Food Science and Engineering College, Beijing University of Agriculture, Beijing Engineering Laboratory of Probiotics Key Technology Development, Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides, Beijing 102206,China)

机构地区：[1]北京农学院食品科学与工程学院,微生态制剂关键技术开发北京市工程实验室,食品质量与安全北京实验室,农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206 [2]富乐顿生物工程科技(北京)有限公司,北京100022 [3]解放军总医院南楼呼吸科,北京100853

出　　处：《航天医学与医学工程》2019年第2期122-127,共6页Space Medicine & Medical Engineering

基　　金：国家自然科学基金青年科学基金项目(31201310);国家重点基础研究发展计划资助(2014CB744400);科技部重大专项项目(2015ZX09J15102-003);军事医学重大工程项目(BWS17C030)

摘　　要：目的研究神舟十号和十一号飞船两次搭载后筛选得到的高产胞外多糖(extracellular polysaccharide,EPS)空间诱变植物乳杆菌SS18-119的抗脂质过氧化功效。方法 48只SPF级大鼠随机分为高脂血症模型组和SS18-119菌株低、中、高剂量组,各组均饲喂高脂饲料以建立模型。模型组灌胃生理盐水,低、中、高剂量分别按照10~7、10~8、10~9 CFU/mL SS18-119灌胃8周。实验结束后,取大鼠肝脏组织进行病理学实验,测定大鼠血清和肝脏脂质含量、肝脏抗氧化相关指标。结果造模第28天,各组大鼠血脂含量显著升高,表明高脂血症模型建立成功。与模型组相比,高剂量组血清ALT活性显著降低(P<0.05),肝脏总胆固醇(total cholesterol,TC)含量显著降低46.6%(P<0.05),甘油三酯(triglyceride,TG)含量显著降低38.1%(P<0.01),低密度脂蛋白-C(low density lipoprotein,LDL-C)含量显著降低41.3%(P<0.05)。与模型组相比,高剂量组血清丙二醛(malondialdehyde,MDA)含量显著降低45.6%(P<0.01),超氧化物歧化酶(superoxide dismutase,SOD)活力显著提升6.9%(P<0.01),谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力显著提升2.6%(P<0.05),总抗氧化能力(total antioxidant capacity,T-AOC)显著提升5.6%(P<0.01)。油红O染色显示SS18-119菌株能够明显抑制脂肪肝,HE染色显示试验组肝脏病变程度明显减轻。结论空间诱变植物乳杆菌SS18-119高剂量组具有保护大鼠肝脏、抑制脂肪肝形成及较强的抗脂质过氧化功效。Objective To determine the anti-lipid peroxidation effect of SS18-119,a space-mutated lactobacillus plantarum carried twice by Shenzhou 10 and 11 spacecraft,with high yield of extracellular polysaccharides and excellent in vitro antioxidant activity,in vivo with hyperlipemia(HLP)rat model.Methods Rats were randomly divided into model group,low,medium and high test substance groups of space-mutated lactobacillus plantarum SS18-119.The model group was given gastric physiological saline,and the low,medium and high test substance groups were given gastric gavage 107,108,and109 CFU/mL space mutagenesis plant lactobacillus SS18-119 strains.All groups were fed high-fat diet to establish hyperlipidemia model for 8 weeks.Serum,liver lipids and liver antioxidant related indexes were measured.Results There was a significant difference in blood lipid content in each group on 28 d compared with that on 0 d,indicating that the model of hyperlipidemia was successfully established.At the 8 th week,high test substance groups serum alanine aminotransferase activity was significantly lower than that of the model group(P<0.05).Compared with model group,liver total cholesterol in high test substance group was significantly reduced by 46.6%(P<0.05),and triglycerides were significantly reduced by 38.1%(P<0.01),low density lipoprotein-C content decreased significantly by41.3%(P<0.05).Compared with model group,serum MDA content in high test substance group decreased significantly by 45.6%(P<0.01),superoxide dismutase activity significantly increased by 6.9%(P<0.01),glutathione peroxidase activity significantly increased by 2.6%(P<0.05),the total antioxidant capacity significantly increased by 5.6%(P<0.01).Oil red O staining result showed that the high dose of test substance had the best inhibition of fatty liver,the HE staining result showed that the degree of liver lesions was significantly reduced in experimal groups.Conclusion The high dose of test substance from space mutagenesis lactobacillus plantarum SS18-119 has the ability of pr

关 键 词：空间诱变植物乳杆菌 胞外多糖 高脂血症 抗脂质过氧化 

分 类 号：R851.8[医药卫生—航空、航天与航海医学]