轻度认知功能障碍患者的肠道菌群特征  

Gut microbial characteristics of people with mild cognitive impairment

作　　者：曾秀丽 谭楚红 吴齐恒[1] 何彦[2] 尹恝[1] 朱佳佳[1] ZENG Xiuli;TAN Chuhong;WUQiheng;HE Yan;YIN Jia;ZHU Jiajia(Department of Neurology,Nanfang Hospital,Southern Medical University,NO.1838,North Guangzhou Avenue,Guangzhou,510515,China)

机构地区：[1]广东省广州市南方医科大学南方医院神经内科,广州510515 [2]广东省广州市南方医科大学珠江医院检验医学部

出　　处：《中国神经精神疾病杂志》2019年第3期129-134,共6页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金项目(编号:81671171);广东省自然基金项目(编号:2017A030313821)

摘　　要：目的探究轻度认知功能障碍患者的肠道菌群特征和危险因素。方法纳入28例轻度认知功能障碍患者,年龄、性别和教育水平相当的65例认知功能正常者作为对照组。对所有受试者进行颈动脉彩超检查,采集空腹抗凝血和新鲜粪样,提取受试者粪便样本中的细菌DNA并进行16S rRNA测序,分析轻度认知功能障碍的危险因素。结果和对照组相比,轻度认知功能障碍组肠道菌群显著改变(P<0.05),主要表现为肠道内变形菌门、互养菌门、乳酸杆菌等细菌富集和费氏刺骨鱼菌的减少。多元逻辑回归分析提示,高回声斑块数量(OR=11.511, 95%CI:1.406～94.226, P=0.023)和乳酸杆菌高丰度(OR=4.894, 95%CI:1.090～21.966, P=0.038)与轻度认知功能障碍显著相关。结论肠道内乳酸杆菌高丰度可能是轻度认知功能障碍潜在的危险因素。Objective To investigate the gut microbial characteristics and risk factors in people with mild cognitive impairment.Methods Twenty-eight people with mild cognitive impairment and 65 age-,gender-as well as education-matched controls were recruited.Carotid artery ultrasonography was conducted and fasting anticoagulant blood and fresh fecal samples were collected on all subjects.Total bacterial DNA was extracted from the stool samples and amplified through real-time quantitative polymerase chain reaction(qPCR).Next generation analysis of 16S rRNA was performed to achieve genomic sequences using the Illumina Hiseq 2500 platform.Results Compared with the control group,mild cognitive impairment group exhibited greater microbial diversity(Bray Curtis distance,Adonis test,P<0.05),which were characterized by enrichment of proteobacteria,Synergistetes,lactobacillus,and depletion of Epulopiscium.Age,creatinine,average CCAIMT,Crouse score,and plaque numbers were associated with cognitive impairment.Multiple logistic regression analysis revealed that the number of hyperechoic plaques(OR=11.511,95% CI:1.406~94.226,P=0.023)and the abundance of lactobacillus(OR=4.894,95% CI:1.090~21.966,P=0.038)were the important risk factors for mild cognitive impairment.Conclusion Higher abundance of lactobacillus in the gut holds potential as novel risk factor of people with mild cognitive impairment.

关 键 词：认知功能障碍 肠道菌群 16S RRNA 乳酸杆菌 危险因素 

分 类 号：R749.1[医药卫生—神经病学与精神病学]