高效液相色谱法测定阿萨伊冻干粉中矢车菊素-3-O-葡萄糖苷的含量  

Determination of Cyanidin-3-O-Glucoside Content in A?aí Freeze-Dried Powder by High-Performance Liquid Chromatography

作　　者：王玉杰[1] 李伟[1] 王林元[1] 汤如莹 瞿研[1] 贺成[1] 张建军[1] WANG Yu-jie;LI Wei;WANG Lin-yuan;TANG Ru-ying;QU Yan;HE Cheng;ZHANG Jian-jun(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《食品工业科技》2019年第9期258-263,共6页Science and Technology of Food Industry

基　　金：北京中医药大学中青年教师自主课题(2018-JYBZZ-JS028)

摘　　要：目的:建立以HPLC-紫外检测器测定阿萨伊冻干粉中矢车菊素-3-O-葡萄糖苷含量的方法。方法:分别采用水和1%盐酸水溶液为溶剂,使矢车菊素-3-O-葡萄糖苷分别呈中性醌式碱和烊阳离子构型,比较两种构型对测定波长、色谱峰分离度、供试品制备方法、响应因子和标准曲线,以及对含量测定结果的影响。结果:采用烊阳离子构型,即供试品和对照品用1%盐酸水溶液为溶剂,以DIKMA C_(18)(4.6 mm×250 mm×5μm)柱为分析柱,乙腈-0.5%磷酸溶液(12∶88,V/V)为流动相,流速为1 mL/min,检测波长为520 nm。矢车菊素-3-O-葡萄糖苷烊阳离子构型在0.002419～0.077412 mg/mL范围呈线性关系,y=37029.5438x-7.6051(R^2=0.9999),回收率为98.31%(RSD%=1.44%),3批阿萨伊冻干粉中矢车菊素-3-O-葡萄糖苷的含量为0.1158～0.1663 mg/g。结论:采用矢车菊素-3-O-葡萄糖烊阳离子构型,以HPLC-紫外检测器测定阿萨伊冻干粉中矢车菊素-3-O-葡萄糖苷含量的方法操作简便、灵敏、结果准确、重复性好。Objective:To establish a high-performance liquid chromatography(HPLC)method for simultaneous determination of cyanidin-3-O-glucoside in Acaífreeze-dried powder.Methods:Water and 1%hydrochloric acid aqueous solution were used as solvents respectively.The cyanidin-3-O-glucoside was in the form of Neutral quinonoidal anhydrobase and flavylium cation,the effects of the two forms on the determination wavelength,chromatographic peak resolution,preparation method,response factor,standard curve and determination results were compared.Results:Using a flavylium cation form,the test sample and the reference substance were treated with a 1%hydrochloric acid aqueous solution as a solvent.The separation was performed on a DIKMA C18 chromatography column(4.6 mm×250 mm×5μm),acetonitrile-water with 0.5%phosphoric(12∶88,V/V)was used as mobile phase at a flow rate of 1 mL/min,the detection wavelength was 520 nm.The developed regression equation for the form of cyanidin-3-O-glucoside flavylium cation standard was:y=37029.5438x-7.6051(R^2=0.9999).A good linearity was shown in the range of 0.002419~0.07741 mg/mL.The average recoveries were 98.31%(RSD%=1.44%).The contents of cyanidin-3-O-glucoside in the three batches of samples were 0.1158~0.1663 mg/g.Conclusion:Using the form of cyanidin-3-O-glucoside flavylium cation,the method was easy to operate,sensitive,accurate,and had better reproducibility for the determination of cyanidin-3-O-glucoside in Acaífreeze-dried powder by HPLC.

关 键 词：HPLC-紫外检测器 阿萨伊冻干粉 矢车菊素-3-O-葡萄糖苷 中性醌式碱和烊阳离子构型 

分 类 号：TS207.3[轻工技术与工程—食品科学]