大豆肽下调Notch 1信号通路抑制哮喘大鼠气道炎症反应  

Soybean peptide inhibit the airway inflammation of rats with bronchial asthma via down-regulating the Notch 1 signaling pathway

作　　者：张玲[1] 徐宛玲[1] 崔明辰[1] 徐松涛[1] 李盘欣 黄亚男 马永超[1] ZHANG Ling;XU Wan-ling;CUI Ming-chen;XU Song-tao;LI Pan-xin;HUANG Ya-nan;MA Yong-chao(Luohe Medical College,Tumor Occurrence and Prevention Research Innovation Team of Henan,Luohe,Henan 462002,China)

机构地区：[1]漯河医学高等专科学校河南省肿瘤发生与防治创新型科技团队,河南漯河462002 [2]河南省南街村(集团)有限公司,河南临颍462600

出　　处：《现代预防医学》2019年第9期1658-1662,共5页Modern Preventive Medicine

基　　金：河南省科技攻关计划项目(No.152102210209;No.152102310219);河南省博士后基金项目(No.2014102)

摘　　要：目的探讨大豆肽(soybean peptide,SP)通过下调Notch 1信号通路防治哮喘大鼠气道炎症反应的作用机制。方法将60只SD雄性大鼠随机分为正常对照组、哮喘模型组、地塞米松(Dexamethasone,Dex)0.5 mg/kg组及大豆肽600、300及150 mg/kg剂量组。采用卵清蛋白(Ovalbumin,OVA)+氢氧化铝(Aluminum hydroxide,Al(OH)_3)法复制大鼠哮喘动物模型,同时给予相应剂量的大豆肽进行干预,每天给药1次,连续7 d。末次给药24 h后收集大鼠肺泡灌洗液(broncho-alveolar lavge fluid,BALF),进行白细胞分类计数;取大鼠右下叶肺组织常规HE染色,观察肺组织病理学改变及气道重塑变化;采用逆转录PCR和Western-blot法检测大鼠肺组织Notch 1、Jagged 1、Hes 1及NF-κB mRNA和蛋白表达水平。结果与哮喘模型组比较,大豆肽600 mg/kg剂量组大鼠BALF中的中性粒细胞、淋巴细胞及嗜酸性粒细胞计数显著降低(P<0.01);大鼠肺组织病理损伤较模型组明显改善,炎性细胞浸润明显减少;大鼠肺组织Notch 1、Jagged 1、Hes 1及NF-κB mRNA和蛋白表达水平明显下调(P<0.05,P<0.01)。结论大豆肽对哮喘大鼠炎症反应具有防治作用,其机制与下调Notch 1/Jagged 1/Hes 1/NF-κB信号通路有关。Objective To observe the inhibition inflammation effect of soybean peptide ( SP) on the airway of rats with bronchial asthma, and to explore its underling mechanism about Notch 1 signaling pathway. Methods Sixty SD rats were divided into normal control group,asthma model group,dexamethasone 0. 5 mg /kg group,and soybean peptide 600,300 and 150 mg /kg group,randomly. The asthma rat model was established by ovalbumin + Al ( OH) 3 sensitization,and the different dose of SP were given the model rats, once a day for 7 days. After 24 h in the last administration, the bronchoalveolar lavage fluid ( BALF) was collected to detect the classified and counter of leucocyte. The pathological changes of lung tissue and airway remodeling were observed by H&E staining. The mRNA and protein expression levels of Notch 1,Jagged 1,Hes 1and NF -κB of lung tissue were analyzed and determined by reverse transcription - polymerase chain reaction ( RT - PCR) and Western blotting methods. Results Compare with the asthma model group, the SP ( 600 mg /kg) could decrease the neutrophil, lymphocyte and eosinophils in BALF of asthma rats ( P < 0. 01), relief the pathological damage of lung tissue and down - regulate the mRNA and protein expression levels of Notch 1,Jagged 1,Hes 1 and NF -κB of lung tissue ( P < 0. 05,P < 0. 01). Conclusion SP has the effect of anti - inflammation in asthma rats,and its mechanism may be related to down - regulate the Notch 1 /Jagged 1 /Hes 1 /NF -κB signaling pathway.

关 键 词：大豆肽 哮喘 NOTCH1 炎症 

分 类 号：R13[医药卫生—劳动卫生]